Cytokeratin 5+6 Mouse Monoclonal Antibody [A2A12]
cat.: EM1901-07
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human, Rat
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: A2A12
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 60/62 kDa
Isotype: IgG1
Immunogen: Synthetic peptide within Human Cytokeratin 5 aa 340-380.
Positive control: HT-29 cell lysate, A549 cell lysate, MCF7 cell lysate, HeLa cell lysate, human tonsil tissue, human skin tissue, human lung adenocarcinoma tissue, rat skin tissue, A431.
Subcellular location: Cytoskeleton.
Recommended Dilutions:
  WB
  ChICC/IHC-P
  FC

1:1,000-1:5,000
1:100-1:500
1:50-1:100
Uniprot #: SwissProt: P02538 Human | P04259 Human | P13647 Human | Q6P6Q2 Rat | Q4FZU2 Rat
Alternative names: 58 kDa cytokeratin CK-5 CK5 Cytokeratin-5 Cytokeratin5 DDD DDD1 EBS2 epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types K2C5_HUMAN K5 keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types) Keratin 5 Keratin keratin complex 2, basic, gene 5 keratin, type II cytoskeletal 5 Keratin-5 Keratin5 KRT 5 Krt5 KRT5A type II cytoskeletal 5 Type-II keratin Kb5 CK6 CK 6A CK 6B CK 6C CK 6D CK 6E CK-6B CK-6C CK-6E Cytokeratin 6a Cytokeratin 6B Cytokeratin 6C Cytokeratin 6D Cytokeratin 6E Cytokeratin-6B Cytokeratin-6C Cytokeratin-6E K2C6C_HUMAN K6a keratin K6b keratin K6C K6c keratin K6d keratin K6e keratin Keratin Keratin K6h Keratin type II cytoskeletal 6A Keratin type II cytoskeletal 6B Keratin type II cytoskeletal 6C Keratin type II cytoskeletal 6D Keratin type II cytoskeletal 6E Keratin-6C KRT6A KRT6B KRT6C KRT6D KRT6E type II......
Images
EM1901-07_1.jpg Fig1: Western blot analysis of Cytokeratin 5+6 on different lysates with Mouse anti-Cytokeratin 5+6 antibody (EM1901-07) at 1/1,000 dilution.

Lane 1: HT-29 cell lysate
Lane 2: A549 cell lysate
Lane 3: MCF7 cell lysate
Lane 4: HeLa cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 60/62 kDa
Observed band size: 50 kDa

Exposure time: 24 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-07) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
EM1901-07_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 5+6 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-07, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-07_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human skin tissue with Mouse anti-Cytokeratin 5+6 antibody (EM1901-07) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-07) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-07_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue with Mouse anti-Cytokeratin 5+6 antibody (EM1901-07) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-07) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-07_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat skin tissue with Mouse anti-Cytokeratin 5+6 antibody (EM1901-07) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-07) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-07_6.jpg Fig6: Flow cytometric analysis of Cytokeratin 5+6 was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-07, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.