EM1901-10

SERPINC1 Mouse Monoclonal Antibody [15F2]

cat.: EM1901-10

Product Type:	Mouse monoclonal IgG1, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IHC-P, FC
Clonality:	Monoclonal
Clone number:	15F2

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	2ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	53 kDa
Isotype:	IgG1
Immunogen:	Recombinant protein within Human SERPINC1 aa 150-370 / 464.
Positive control:	HL-60 cell lysate, HepG2 cell lysate, SiHa cell lysate, rat testis tissue, human lung carcinoma tissue, human liver tissue, human liver carcinoma tissue, mouse kidney tissue, HCT116 cell.
Subcellular location:	Extracellular space or secreted.
Recommended Dilutions: WB ICC/IHC-P FC	1:500-1:2,000 1:50-1:200 1:50-1:100
Uniprot #:	SwissProt: P01008 Human \| P32261 Mouse \| Q5M7T5 Rat
Alternative names:	ANT3_HUMAN Antithrombin Antithrombin III Antithrombin-III AntithrombinIII AT 3 AT III AT3 AT3D ATIII Heparin cofactor I MGC22579 Serine (or cysteine) proteinase inhibitor clade C (antithrombin) member 1 Serine cysteine proteinase inhibitor clade C member 1 Serine proteinase inhibitor clade C member 1 Serpin C1 Serpin family C member 1 Serpin peptidase inhibitor clade C (antithrombin) member 1 SERPINC1 THPH7

Images

	Fig1: Western blot analysis of SERPINC1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-10, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HL-60 cell lysate Lane 2: HepG2 cell lysate Lane 3: SiHa cell lysate
	Fig2: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-SERPINC1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (EM1901-10, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig3: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-SERPINC1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (EM1901-10, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-SERPINC1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (EM1901-10, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig5: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-SERPINC1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (EM1901-10, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-SERPINC1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (EM1901-10, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig7: Flow cytometric analysis of SERPINC1 was done on HCT116 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-10, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.