MCM7 Mouse Monoclonal Antibody [15E1]
cat.: EM1901-12
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | 15E1 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 81 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human MCM7 aa 516-719 / 719. |
| Positive control: | HL-60 cell lysate, HEK-293 cell lysate, HeLa cell lysate, Jurkat cell lysate, SW480 cell lysate, LNCaP cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, PC-12 cell lysate, COS-1 cell lysate, human tonsil tissue, human esophagus tissue, SH-SY5Y. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB ICC/IHC-P FC |
1:500-1:2,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P33993 Human | Q61881 Mouse Entrez Gene: 288532 Rat |
| Alternative names: | CDABP0042 CDC 47 CDC47 CDC47 homolog Cdc47, S. cerevisiae, homolog of DNA replication licensing factor MCM7 Homolog of S. cerevisiae Cdc47 MCM 2 MCM 7 MCM2 MCM2, formerly Mcm7 MCM7 minichromosome maintenance deficient 7 MCM7_HUMAN Minichromosome Maintainence 7 Minichromosome maintainence, S. cerevisiae, homolog of Minichromosome maintenance complex component 7 Minichromosome maintenance deficient 7 Minichromosome maintenance protein 7 P1.1 MCM3 P1.1-MCM3 P1CDC47 P85MCM PNAS 146 PNAS146 PPP1R104 Protein phosphatase 1, regulatory subunit 104 |
Images
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Fig1:
Western blot analysis of MCM7 on different lysates with Mouse anti-MCM7 antibody (EM1901-12) at 1/1,000 dilution. Lane 1: HL-60 cell lysate Lane 2: HEK-293 cell lysate Lane 3: HeLa cell lysate Lane 4: Jurkat cell lysate Lane 5: SW480 cell lysate Lane 6: LNCaP cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: C2C12 cell lysate Lane 9: PC-12 cell lysate Lane 10: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 81 kDa Observed band size: 81 kDa Exposure time: 51 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-12) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MCM7 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-12, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-MCM7 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-12, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Flow cytometric analysis of MCM7 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-12, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.