Cathepsin D Mouse Monoclonal Antibody [13F2]
cat.: EM1901-15
Product Type: Mouse monoclonal IgG2b, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: 13F2
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein G affinity purified.
Molecular weight: Predicted band size: 45 kDa
Isotype: IgG2b
Immunogen: Recombinant protein within Human Cathepsin D aa 1-412 / 412.
Positive control: MCF7 cell lysate, U-937 cell lysate, SK-Br-3 cell lysate, HepG2 cell lysate, A431 cell lysate, human liver tissue lysate, human lung tissue, human liver tissue, mouse brain tissue, rat cerebellum tissue, SKOV-3.
Subcellular location: Lysosome, extracellular space, melanosome.
Recommended Dilutions:
  WB
  IHC-P
  FC
1:1,000-1:5,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P07339 Human | P18242 Mouse | P24268 Rat
Alternative names: CatD CATD_HUMAN Cathepsin D Cathepsin D heavy chain CD Ceroid lipofuscinosis neuronal 10 CLN10 CPSD ctsd Epididymis secretory sperm binding protein Li 130P HEL S 130P Lysosomal aspartyl peptidase Lysosomal aspartyl protease MGC2311
Images
EM1901-15_1.jpg Fig1: Western blot analysis of Cathepsin D on different lysates with Mouse anti-Cathepsin D antibody (EM1901-15) at 1/1,000 dilution.

Lane 1: MCF7 cell lysate (20 µg/Lane)
Lane 2: U-937 cell lysate (20 µg/Lane)
Lane 3: SK-Br-3 cell lysate (20 µg/Lane)
Lane 4: HepG2 cell lysate (20 µg/Lane)
Lane 5: A431 cell lysate (20 µg/Lane)
Lane 6: Human liver tissue lysate (40 µg/Lane)

Predicted band size: 45 kDa
Observed band size: 45/28 kDa

Exposure time: 3 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-15) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
EM1901-15_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Cathepsin D antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-15, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-15_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Cathepsin D antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-15, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-15_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Cathepsin D antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-15, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-15_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-Cathepsin D antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-15, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-15_6.jpg Fig6: Flow cytometric analysis of Cathepsin D was done on SKOV-3 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-15, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.