NM23 Mouse Monoclonal Antibody [13C2]
cat.: EM1901-18
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | 13C2 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 17 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within Human NM23 aa 10-30. |
| Positive control: | HEK-293 cell lysate, MCF7 cell lysate, Jurkat cell lysate, Raji cell lysate, HeLa cell lysate, K-562 cell lysate, A549 cell lysate, A431 cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, mouse kidney tissue lysate, mouse liver tissue lysate, rat brain tissue lysate, mouse kidney tissue, HeLa, NIH/3T3, C6. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:2,000-1:5,000 1:2,000 1:100 |
| Uniprot #: | SwissProt: P15531 Human | P15532 Mouse | Q05982 Rat |
| Alternative names: | AWD AWD, drosophila, homolog of GAAD Granzyme A activated DNase Granzyme A-activated DNase GZMA activated DNase Metastasis inhibition factor NM23 NB NBS NDK A NDKA NDKA_HUMAN NDP kinase A NDPK-A NDPKA NM23 NM23 long variant, included nm23-H1 NM23-M1 NM23H1B, included NME/NM23 nucleoside diphosphate kinase 1 Nme1 NME1-NME2 spliced read-through transcript, included Non-metastatic cells 1, protein (NM23A) expressed in Nonmetastatic cells 1, protein expressed in Nonmetastatic protein 23 Nonmetastatic protein 23, homolog 1 Nucleoside diphosphate kinase A Tumor metastatic process-associated protein |
Images
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Fig1:
Western blot analysis of NM23 on different lysates with Mouse anti-NM23 antibody (EM1901-18) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate (20 µg/Lane) Lane 2: MCF7 cell lysate (20 µg/Lane) Lane 3: Jurkat cell lysate (20 µg/Lane) Lane 4: Raji cell lysate (20 µg/Lane) Lane 5: HeLa cell lysate (20 µg/Lane) Lane 6: K-562 cell lysate (20 µg/Lane) Lane 7: A549 cell lysate (20 µg/Lane) Lane 8: A431 cell lysate (20 µg/Lane) Lane 9: HepG2 cell lysate (20 µg/Lane) Lane 10: NIH/3T3 cell lysate (20 µg/Lane) Lane 11: C6 cell lysate (20 µg/Lane) Lane 12: Mouse kidney tissue lysate (40 µg/Lane) Lane 13: Mouse liver tissue lysate (40 µg/Lane) Lane 14: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 17 kDa Observed band size: 17/20 kDa Exposure time: 1 minute 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-18) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-NM23 antibody (EM1901-18) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-18) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunocytochemistry analysis of HeLa cells labeling NM23 with Mouse anti-NM23 antibody (EM1901-18) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-NM23 antibody (EM1901-18) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
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Fig4:
Immunocytochemistry analysis of NIH/3T3 cells labeling NM23 with Mouse anti-NM23 antibody (EM1901-18) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-NM23 antibody (EM1901-18) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
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Fig5:
Immunocytochemistry analysis of C6 cells labeling NM23 with Mouse anti-NM23 antibody (EM1901-18) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-NM23 antibody (EM1901-18) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
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