Myeloperoxidase Mouse Monoclonal Antibody [A1F2]
cat.: EM1901-19
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC, IF-Cell, IF-Tissue
Clonality: Monoclonal
Clone number: A1F2
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein G affinity purified.
Molecular weight: Predicted band size: 84 kDa
Isotype: IgG1
Immunogen: Recombinant protein within human Myeloperoxidase aa 550-745.
Positive control: HL-60 cell lysates, human colon tissue, human colon cancer tissue, human tonsil tissue, mouse colon tissue, rat colon tissue, HL-60.
Subcellular location: Lysosome.
Recommended Dilutions:
  WB
  IHC-P
  FC
  IF-Cell
  IF-Tissue
1:2,000-1:5,000
1:5,000-1:10,000
1:50-1:100
1:100
1:1,000-1:2,000
Uniprot #: SwissProt: P05164 Human | P11247 Mouse
Entrez Gene: 303413 Rat
Alternative names: 84 kDa myeloperoxidase 89 kDa myeloperoxidase EC 1.11.1.7 EC1.11.2.2 fj80f04 MPO mpx myeloid-specific peroxidase Myeloperoxidase Myeloperoxidase heavy chain Myeloperoxidase light chain PERM_HUMAN wu:fj80f04
Images
EM1901-19_1.jpg Fig1: Western blot analysis of Myeloperoxidase on HL-60 cell lysates with Mouse anti-Myeloperoxidase antibody (EM1901-19) at 1/1,000 dilution.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 84 kDa
Observed band size: 84/55/37 kDa

Exposure time: 24 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-19) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
EM1901-19_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human colon tissue with Mouse anti-Myeloperoxidase antibody (EM1901-19) at 1/10,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-19) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-19_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-Myeloperoxidase antibody (EM1901-19) at 1/5,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-19) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-19_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-Myeloperoxidase antibody (EM1901-19) at 1/5,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-19) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-19_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Mouse anti-Myeloperoxidase antibody (EM1901-19) at 1/10,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-19) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-19_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded rat colon tissue with Mouse anti-Myeloperoxidase antibody (EM1901-19) at 1/5,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-19) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-19_7.jpg Fig7: Flow cytometric analysis of Myeloperoxidase was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-19, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
EM1901-19_8.jpg Fig8: Immunocytochemistry analysis of HL-60 cells labeling Myeloperoxidase with Mouse anti-Myeloperoxidase antibody (EM1901-19) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Myeloperoxidase antibody (EM1901-19) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.