Myeloperoxidase Mouse Monoclonal Antibody [A1F3]
cat.: EM1901-20
| Product Type: | Mouse monoclonal IgG2a, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | A1F3 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 84 kDa |
| Isotype: | IgG2a |
| Immunogen: | Recombinant protein within human Myeloperoxidase aa 550-745. |
| Positive control: | HL-60 (Human acute promyelocytic leukemia cell) cell lysates, human tonsil tissue, human colon tissue, human colon carcinoma tissue, HL-60. |
| Subcellular location: | Lysosome. |
| Recommended Dilutions:
WB IHC-P FC |
1:2,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P05164 Human |
| Alternative names: | 84 kDa myeloperoxidase 89 kDa myeloperoxidase EC 1.11.1.7 EC1.11.2.2 fj80f04 MPO mpx myeloid-specific peroxidase Myeloperoxidase Myeloperoxidase heavy chain Myeloperoxidase light chain PERM_HUMAN wu:fj80f04 |
Images
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Fig1:
Western blot analysis of Myeloperoxidase on HL-60 (Human acute promyelocytic leukemia cell) cell lysate with Mouse anti-Myeloperoxidase antibody (EM1901-20) at 1/2,000 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 59 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: EM1901-20, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 84 kDa Observed band size: 84/55/37 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Myeloperoxidase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-20, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Myeloperoxidase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-20, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Myeloperoxidase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-20, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Flow cytometric analysis of Myeloperoxidase was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-20, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.