Anti-Myeloperoxidase antibody [A1F3]
cat.: EM1901-20
Product Type: Mouse monoclonal IgG2a, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: A1F3
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/mL
Purification: Protein G purified.
Molecular weight: 51 kDa
Isotype: IgG2a
Immunogen: Recombinant protein within human Myeloperoxidase aa 550-745.
Positive control: HL-60 cell, human tonsil tissue, human colon tissue, human colon carcinoma tissue.
Subcellular location: Lysosome.
Recommended Dilutions:
  WB
  IHC-P
  FC

1:500-1:1,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P05164 Human
Alternative names: 84 kDa myeloperoxidase antibody 89 kDa myeloperoxidase antibody EC 1.11.1.7 antibody EC1.11.2.2 antibody fj80f04 antibody MPO antibody mpx antibody myeloid-specific peroxidase antibody Myeloperoxidase antibody Myeloperoxidase heavy chain antibody Myeloperoxidase light chain antibody PERM_HUMAN antibody wu:fj80f04 antibody
Images
EM1901-20_1.jpg Fig1: Western blot analysis of Myeloperoxidase on HL-60 lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-20, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
EM1901-20_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Myeloperoxidase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-20, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-20_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Myeloperoxidase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-20, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-20_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Myeloperoxidase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-20, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-20_5.jpg Fig5: Flow cytometric analysis of Myeloperoxidase was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-20, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.