Thymidine Phosphorylase Mouse Monoclonal Antibody [A1A10]
cat.: EM1901-22
| Product Type: | Mouse monoclonal IgG2a, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | A1A10 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa |
| Isotype: | IgG2a |
| Immunogen: | Recombinant protein within Human Thymidine Phosphorylase aa 19-239 / 482. |
| Positive control: | THP-1 cell lysate, A549 cell lysate, A431 cell lysate, HeLa cell lysate, human liver carcinoma tissue, human spleen tissue, SiHa, mouse spleen tissue, rat spleen tissue. |
| Subcellular location: | Cytosol. |
| Recommended Dilutions:
WB IHC-P FC IF-Cell |
1:500-1:1,000 1:200-1:10,000 1:50-1:100 1:50-1:100 |
| Uniprot #: | SwissProt: P19971 Human | Q99N42 Mouse | Q5FVR2 Rat |
| Alternative names: | ECGF 1 ECGF ECGF1 Endothelial cell growth factor 1 Endothelial cell growth factor 1 platelet derived Endothelial cell growth factor, platelet-derived Gliostatin hPD ECGF MEDPS1 MNGIE MTDPS1 PD ECGF PD-ECGF PDECGF PDEGF Platelet derived endothelial cell growth factor Platelet derived endothelial growth factor Platelet-derived endothelial cell growth factor TdRPase Thymidine phosphorylase TP Tymp TYPH_HUMAN |
Images
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Fig1:
Western blot analysis of Thymidine Phosphorylase on different lysates with Mouse anti-Thymidine Phosphorylase antibody (EM1901-22) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: A549 cell lysate Lane 3: A431 cell lysate Lane 4: HeLa cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-22) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-Thymidine Phosphorylase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-22, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Thymidine Phosphorylase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-22, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Flow cytometric analysis of Thymidine Phosphorylase was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-22, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Mouse anti-Thymidine Phosphorylase antibody (EM1901-22) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-22) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Mouse anti-Thymidine Phosphorylase antibody (EM1901-22) at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-22) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.