Anti-CD10 antibody [A1G3]
cat.: EM1901-24
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: A1G3
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/mL
Purification: Protein G affinity purified.
Molecular weight: 86 kDa
Isotype: IgG1
Immunogen: Synthetic peptide within human CD10 aa 200-300.
Positive control: Daudi cell lysates, human prostate tissue, human kidney tissue, 293.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB
  IHC-P
  FC

1:500-1:2,000
1:200-1:1,000
1:50-1:100
Uniprot #: SwissProt: P08473 Human
Alternative names: Atriopeptidase antibody CALLA antibody CD10 antibody CD10 antigen antibody Common acute lymphocytic leukemia antigen antibody DKFZp686O16152 antibody EC 3.4.24.11 antibody Enkephalinase antibody EPN antibody Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase) antibody Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10) antibody Membrane metallo endopeptidase antibody Membrane metallo endopeptidase variant 1 antibody Membrane metallo endopeptidase variant 2 antibody Membrane metalloendopeptidase antibody Membrane metalloendopeptidase neutral endopeptidase enkephalinase antibody Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10 antibody Membrane metalloendopeptidase variant 1 antibody Membrane metalloendopeptidase variant 2 antibody MGC126681 antibody MGC126707 antibody MME antibody NEP antibody NEP_HUMAN antibody Neprilysin antibody neprilysin-390 antibody neprilysin-411 antibody Neutral endopeptidase 24.11 antibody Neutral endopeptidase antibody Neutral endopeptidase, membrane-associated antibody SFE antibody Skin fibroblast elastase antibody
Images
EM1901-24_1.jpg Fig1: Western blot analysis of CD10 on Daudi cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-24, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
EM1901-24_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-CD10 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-24, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-24_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD10 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-24, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-24_4.jpg Fig4: Flow cytometric analysis of CD10 was done on 293 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-24, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.