CD10 Mouse Monoclonal Antibody [A1G3]
cat.: EM1901-24
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IHC-P, FC, IF-Tissue
Clonality: Monoclonal
Clone number: A1G3
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein G affinity purified.
Molecular weight: Predicted band size: 86 kDa
Isotype: IgG1
Immunogen: Synthetic peptide within human CD10 aa 200-300.
Positive control: Daudi cell lysate, Ramos cell lysate, LNCaP cell lysate, mouse kidney tissue lysate, human renal clear cell carcinoma tissue, human kidney tissue, human prostate tissue, 293.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB
  IHC-P
  FC
  IF-Tissue
1:500-1:2,000
1:200-1:1,000
1:50-1:100
1:50
Uniprot #: SwissProt: P08473 Human | Q61391 Mouse
Alternative names: Atriopeptidase CALLA CD10 CD10 antigen Common acute lymphocytic leukemia antigen DKFZp686O16152 EC 3.4.24.11 Enkephalinase EPN Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase) Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10) Membrane metallo endopeptidase Membrane metallo endopeptidase variant 1 Membrane metallo endopeptidase variant 2 Membrane metalloendopeptidase Membrane metalloendopeptidase neutral endopeptidase enkephalinase Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10 Membrane metalloendopeptidase variant 1 Membrane metalloendopeptidase variant 2 MGC126681 MGC126707 MME NEP NEP_HUMAN Neprilysin neprilysin-390 neprilysin-411 Neutral endopeptidase 24.11 Neutral endopeptidase Neutral endopeptidase, membrane-associated SFE Skin fibroblast elastase
Images
EM1901-24_1.jpg Fig1: Western blot analysis of CD10 on different lysates with Mouse anti-CD10 antibody (EM1901-24) at 1/2,000 dilution.

Lane 1: Daudi cell lysate (20 µg/Lane)
Lane 2: Ramos cell lysate (20 µg/Lane)
Lane 3: LNCaP cell lysate (20 µg/Lane)
Lane 4: HT-29 cell lysate (20 µg/Lane) (negative)
Lane 5: Mouse kidney tissue lysate (40 µg/Lane)

Predicted band size: 86 kDa
Observed band size: 100 kDa

Exposure time: 5 minutes; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-24) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
EM1901-24_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human renal clear cell carcinoma tissue with Rabbit anti-CD10 antibody (EM1901-24) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-24) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-24_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-CD10 antibody (EM1901-24) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-24) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-24_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-CD10 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-24, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-24_5.jpg Fig5: Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling CD10 with Mouse anti-CD10 antibody (EM1901-24) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (EM1901-24, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
EM1901-24_6.jpg Fig6: Flow cytometric analysis of CD10 was done on 293 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-24, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.