USP21 Mouse Monoclonal Antibody [16E1]
cat.: EM1901-37
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Monoclonal
Clone number: 16E1
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 63 kDa
Isotype: IgG1
Immunogen: Recombinant protein within Human USP21 aa 303-453 / 565.
Positive control: SH-SY5Y cell lysates, K562 cell lysates, SH-SY5Y , rat seminal vesicle tissue, human liver tissue, human kidney tissue, human small intestine tissue, mouse heart tissue.
Subcellular location: Nucleus, cytoplasm.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:500-1:2,000
1:100
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q9UK80 Human | Q9QZL6 Mouse | B2GUX4 Rat
Alternative names: Deubiquitinating enzyme 21 deubiquitinating enzyme 23 EC 3.1.2.15 ESTM28 MGC3394 NEDD8-specific protease Ubiquitin carboxyl terminal hydrolase 21 ubiquitin carboxyl terminal hydrolase 23 Ubiquitin carboxyl-terminal hydrolase 21 ubiquitin specific peptidase 21 Ubiquitin specific processing protease 21 ubiquitin specific protease 16 ubiquitin specific protease 21 ubiquitin specific protease 23 Ubiquitin thioesterase 21 Ubiquitin thiolesterase 21 ubiquitin thiolesterase 23 ubiquitin-specific protease 23, formerly Ubiquitin-specific-processing protease 21 UBP21_HUMAN USP16 Usp21 USP23 USP23, formerly W53272
Images
EM1901-37_1.jpg Fig1: Western blot analysis of USP21 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-37, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SH-SY5Y cell lysates
Lane 2: K562 cell lysates
EM1901-37_2.jpg Fig2: Immunocytochemistry analysis of SH-SY5Y cells labeling USP21 with Mouse anti-USP21 antibody (EM1901-37) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-USP21 antibody (EM1901-37) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution.
EM1901-37_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat seminal vesicle tissue using anti-USP21 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-37, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-37_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-USP21 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-37, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-37_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-USP21 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-37, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-37_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-USP21 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-37, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-37_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-USP21 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-37, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-37_8.jpg Fig8: Flow cytometric analysis of USP21 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-37, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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