Anti-F13A1 antibody [16D3]
cat.: EM1901-38
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: 16D3
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 83 kDa
Isotype: IgG1
Immunogen: Recombinant protein within human F13A1 aa 250-500.
Positive control: Human placenta tissue lysates, human placenta tissue, A549.
Subcellular location: Secreted, cytoplasm.
Recommended Dilutions:

Uniprot #: SwissProt: P00488 Human
Alternative names: bA525O21.1 (coagulation factor XIII, A1 polypeptide) antibody Coagulation factor XIII A chain antibody Coagulation factor XIII A1 polypeptide antibody Coagulation factor XIII A1 subunit antibody Coagulation factor XIII, A polypeptide antibody Coagulation factor XIIIa antibody F13A antibody F13A_HUMAN antibody F13a1 antibody Factor XIIIA antibody Fibrin stabilizing factor, A subunit antibody Fibrinoligase antibody FSF, A subunit antibody Protein glutamine gamma glutamyltransferase A chain antibody Protein-glutamine gamma-glutamyltransferase A chain antibody TGase antibody Transglutaminase A chain antibody Transglutaminase, plasma antibody Transglutaminase. plasma antibody
EM1901-38_1.jpg Fig1: Western blot analysis of F13A1 on human placenta tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-38, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
EM1901-38_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-F13A1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-38, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-38_3.jpg Fig3: Flow cytometric analysis of F13A1 was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-38, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).