Dynamin 1 Mouse Monoclonal Antibody [A1B1]
cat.: EM1901-43
Product Type: Mouse monoclonal IgM, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: IHC-P, WB, IF-Cell
Clonality: Monoclonal
Clone number: A1B1
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 97 kDa
Isotype: IgM
Immunogen: Recombinant protein within human Dynamin 1 aa 500-800.
Positive control: Rat brain tissue, mouse brain, SiHa, mouse brain tissue lysate, rat brain tissue lysate.
Subcellular location: Cytoskeleton, cytoplasm.
Recommended Dilutions:
  IHC-P
  WB
  IF-Cell

1:1,000
1:1,000
1:50
Uniprot #: SwissProt: Q05193 Human | P39053 Mouse | P21575 Rat
Alternative names: B dynamin D100 DNM 1 DNM DNM1 DYN1_HUMAN Dynamin Dynamin-1 Dynamin1
Images
EM1901-43_1.jpg Fig1: Western blot analysis of Dynamin 1 on different lysates with Mouse anti-Dynamin 1 antibody (EM1901-43) at 1/1,000 dilution.

Lane 1: Rat brain tissue lysate
Lane 2: Mouse brain tissue lysate

Lysates/proteins at 40 µg/Lane.

Predicted band size: 97 kDa
Observed band size: 100 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-43) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
EM1901-43_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-Dynamin 1 antibody (EM1901-43) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-43) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-43_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-Dynamin 1 antibody (EM1901-43) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-43) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-43_4.jpg Fig4: Immunocytochemistry analysis of SiHa cells labeling Dynamin 1 with Mouse anti-Dynamin 1 antibody (EM1901-43) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Dynamin 1 antibody (EM1901-43) at 1/50 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.