USP28 Mouse Monoclonal Antibody [A1F9]
cat.: EM1901-46
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | A1F9 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 122 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human USP28 aa 509-860 / 1,077. |
| Positive control: | HeLa cell lysate, A431 cell lysate, 293T cell lysate, SW480 cell lysate, LNCaP cell lysate, MDA-MB-231 cell lysate, MDA-MB-468 cell lysate, rat testis tissue lysate. |
| Subcellular location: | Nucleoplasm. |
| Recommended Dilutions:
WB |
1:500-1:2,000 |
| Uniprot #: | SwissProt: Q96RU2 Human | D3ZJ96 Rat |
| Alternative names: | Deubiquitinating enzyme 28 KIAA1515 Ubiquitin carboxyl terminal hydrolase 28 Ubiquitin carboxyl-terminal hydrolase 28 ubiquitin carboxyl-terminal hydrolase 28 variant 1 Ubiquitin specific peptidase 28 Ubiquitin specific processing protease 28 Ubiquitin specific protease 28 Ubiquitin thioesterase 28 Ubiquitin thiolesterase 28 Ubiquitin-specific-processing protease 28 UBP28_HUMAN USP 28 USP28 USP28 protein |
Images
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Fig1:
Western blot analysis of USP28 on different lysates with Mouse anti-USP28 antibody (EM1901-46) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: A431 cell lysate Lane 3: 293T cell lysate Lane 4: SW480 cell lysate Lane 5: LNCaP cell lysate Lane 6: MDA-MB-231 cell lysate Lane 7: MDA-MB-468 cell lysate Lane 8: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 122 kDa Observed band size: 140 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-46) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.