USP28 Mouse Monoclonal Antibody [A1F8]
cat.: EM1901-47
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: A1F8
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein G affinity purified.
Molecular weight: Predicted band size: 122 kDa
Isotype: IgG1
Immunogen: Recombinant protein within Human USP28 aa 509-860 / 1,077.
Positive control: K562 cell lysates, A549 cell lysates.
Subcellular location: Nucleoplasm.
Recommended Dilutions:
  WB

1:500-1:2,000
Uniprot #: SwissProt: Q96RU2 Human
Alternative names: Deubiquitinating enzyme 28 KIAA1515 Ubiquitin carboxyl terminal hydrolase 28 Ubiquitin carboxyl-terminal hydrolase 28 ubiquitin carboxyl-terminal hydrolase 28 variant 1 Ubiquitin specific peptidase 28 Ubiquitin specific processing protease 28 Ubiquitin specific protease 28 Ubiquitin thioesterase 28 Ubiquitin thiolesterase 28 Ubiquitin-specific-processing protease 28 UBP28_HUMAN USP 28 USP28 USP28 protein
Images
EM1901-47_1.jpg Fig1: Western blot analysis of USP28 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-47, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control:
Lane 1: K562 cell lysate
Lane 2: A549 cell lysate
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.