DFNA5 / GSDME Mouse Monoclonal Antibody [A1H2]
cat.: EM1901-49
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | A1H2 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 55 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human DFNA5 aa 34-214 / 496. |
| Positive control: | Hela cell lysates, HepG2 cell lysates, A431, SiHa, SH-SY5Y. |
| Subcellular location: | Cell membrane, cytosol. |
| Recommended Dilutions:
WB IF-Cell FC |
1:500-1:2,000 1:50-1:100 1:50-1:100 |
| Uniprot #: | SwissProt: O60443 Human |
| Alternative names: | 2310037D07Rik 4932441K13Rik Deafness, autosomal dominant 5 Deafness, autosomal dominant 5 protein DFNA5 DFNA5 gene DFNA5_HUMAN Dfna5h EG14210 Fin15 ICERE 1 ICERE-1 Inversely correlated with estrogen receptor expression 1 Non-syndromic hearing impairment protein 5 Nonsyndromic hearing impairment protein |
Images
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Fig1:
Western blot analysis of DFNA5 / GSDME on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-49, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: HepG2 cell lysate |
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Fig2: ICC staining of DFNA5 / GSDME in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1901-49, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: ICC staining of DFNA5 / GSDME in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1901-49, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4: Flow cytometric analysis of DFNA5 / GSDME was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-49, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.