Aspartate Aminotransferase Mouse Monoclonal Antibody [A2D7]
cat.: EM1901-50
Product Type: Mouse monoclonal IgG2b, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: A2D7
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein G affinity purified.
Molecular weight: Predicted band size: 46 kDa
Isotype: IgG2b
Immunogen: Recombinant protein within Human GOT1 aa 16-211 / 413.
Positive control: HepG2 cell lysate, K562 cell lysate, rat testis tissue, human liver tissue, human colon tissue, human colon carcinoma tissue, mouse kidney tissue, SW620.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB
  IHC-P
  FC
1:1,000-1:2,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P17174 Human | P05201 Mouse | P13221 Rat
Alternative names: AATC_HUMAN Aspartate aminotransferase 1 Aspartate aminotransferase Aspartate aminotransferase cytoplasmic Aspartate aminotransferase cytosolic ASTQTL1 cAspAT cCAT cysteine aminotransferase, cytoplasmic cysteine transaminase, cytoplasmic cytoplasmic ec 2.6.1.1 GIG 18 GIG18 Glutamate oxaloacetate transaminase 1 Glutamate oxaloacetate transaminase soluble Glutamic oxaloacetic transaminase 1 soluble GOT 1 GOT1 Growth inhibiting protein 18 SGOT Transaminase A
Images
EM1901-50_1.jpg Fig1: Western blot analysis of Aspartate Aminotransferase on different lysates with Mouse anti-Aspartate Aminotransferase antibody (EM1901-50) at 1/1,000 dilution.

Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-Aspartate Aminotransferase KD cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 43 kDa
Observed band size: 43 kDa

Exposure time: 2 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-50) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
EM1901-50_2.jpg Fig2: Western blot analysis of Aspartate Aminotransferase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-50, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: HepG2 cell lysate
Lane 2: K562 cell lysate
EM1901-50_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Aspartate Aminotransferase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-50, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-50_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Aspartate Aminotransferase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-50, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-50_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Aspartate Aminotransferase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-50, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-50_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Aspartate Aminotransferase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-50, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-50_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Aspartate Aminotransferase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-50, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-50_8.jpg Fig8: Flow cytometric analysis of Aspartate Aminotransferase was done on SW620 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-50, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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