EM1901-53

ERK2 Mouse Monoclonal Antibody [B10-8]

cat.: EM1901-53

Product Type:	Mouse monoclonal IgG1, primary antibodies
Species reactivity:	Human, Mouse
Applications:	WB, IHC-P, FC
Clonality:	Monoclonal
Clone number:	B10-8

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	2ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 41 kDa
Isotype:	IgG1
Immunogen:	Recombinant protein within human ERK2 aa 200-360.
Positive control:	Hela cell lysate, 293T cell lysate, NIH/3T3 cell lysate, K562 cell lysate, human skin tissue, human breast carcinoma tissue, mouse testis tissue, mouse colon tissue, mouse ovary tissue, K562.
Subcellular location:	Nucleus, spindle, centrosome, cytoplasm, caveola.
Recommended Dilutions: WB IHC-P FC	1:500-1:2,000 1:50-1:200 1:50-1:100
Uniprot #:	SwissProt: P28482 Human \| P63085 Mouse
Alternative names:	ERK 2 ERK-2 ERT1 Extracellular Signal Regulated Kinase 2 Extracellular signal-regulated kinase 2 MAP kinase 1 MAP kinase 2 MAP kinase isoform p42 MAPK 1 MAPK 2 Mapk1 MAPK2 Mitogen-activated protein kinase 1 Mitogen-activated protein kinase 2 MK01_HUMAN P38 P40 P41 p42-MAPK P42MAPK PRKM1 PRKM2 protein kinase, mitogen-activated, 1 protein kinase, mitogen-activated, 2 protein tyrosine kinase ERK2

Images

	Fig1: Western blot analysis of ERK2 on different lysates with Mouse anti-ERK2 antibody (EM1901-53) at 1/2,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si ERK2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 41 kDa Observed band size: 41 kDa Exposure time: 30 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-53) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
	Fig2: Western blot analysis of ERK2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-53, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: 293T cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: K562 cell lysate
	Fig3: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-ERK2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (EM1901-53, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ERK2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (EM1901-53, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ERK2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (EM1901-53, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig6: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-ERK2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (EM1901-53, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig7: Immunohistochemical analysis of paraffin-embedded mouse ovary tissue using anti-ERK2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (EM1901-53, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig8: Flow cytometric analysis of ERK2 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-53, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.