Glutathione peroxidase 1 Mouse Monoclonal Antibody [C5-A10]
cat.: EM1901-56
| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, FC, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | C5-A10 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 22 kDa |
| Isotype: | IgG2b |
| Immunogen: | Recombinant protein within Human Glutathione Peroxidase 1 aa 20-203 / 203. |
| Positive control: | HEK-293 cell lysate, HepG2 cell lysate, SH-SY5Y cell lysate, HT-29 cell lysate, human liver tissue lysate, human kidney tissue lysate, THP-1, human liver tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell FC IHC-P |
1:2,000 1:100 1:1:1,000 1:2,000 |
| Uniprot #: | SwissProt: P07203 Human | P11352 Mouse |
| Alternative names: | AL033363 Cellular glutathione peroxidase Glutathione peroxidase 1 Glutathione peroxidase GPx 1 GPx-1 GPX1 GPX1_HUMAN GPXD GSHPx-1 GSHPX1 MGC14399 MGC88245 |
Images
|
Fig1:
Western blot analysis of Glutathione peroxidase 1 on different lysates with Mouse anti-Glutathione peroxidase 1 antibody (EM1901-56) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: SH-SY5Y cell lysate (20 µg/Lane) Lane 4: HT-29 cell lysate (20 µg/Lane) Lane 5: Human liver tissue lysate (40 µg/Lane) Lane 6: Human kidney tissue lysate (40 µg/Lane) Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-56) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of THP-1 cells labeling Glutathione peroxidase 1 with Mouse anti-Glutathione peroxidase 1 antibody (EM1901-56) at 1/100 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Glutathione peroxidase 1 antibody (EM1901-56) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Glutathione peroxidase 1 antibody (EM1901-56) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-56) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Flow cytometric analysis of THP-1 cells labeling Glutathione peroxidase 1. Cells were fixed and permeabilized. Then stained with the primary antibody (EM1901-56, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.