RUVB2 Mouse Monoclonal Antibody [A1E12]
cat.: EM1901-58
Product Type: Mouse monoclonal IgG2b, primary antibodies
Species reactivity: Human, Mouse, Rat, Monkey
Applications: WB, IF-Cell, IHC-P
Clonality: Monoclonal
Clone number: A1E12
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein G affinity purified.
Molecular weight: Predicted band size: 51 kDa
Isotype: IgG2b
Immunogen: Recombinant protein within Human RUVB2 aa 130-321 / 463.
Positive control: HeLa cell lysate, Daudi cell lysate, HepG2 cell lysate, SK-Br-3 cell lysate, 293T cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C6 cell lysate, PC-12 cell lysate, COS-1 cell lysate, mouse testis tissue lysate, rat testis tissue lysate, mouse thymus tissue lysate, rat thymus tissue lysate, HeLa, RAW264.7, human trachea tissue, human placenta tissue, human testis tissue, mouse testis tissue, rat testis tissue.
Subcellular location: Nucleus matrix, nucleoplasm, cytoplasm, membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
1:500-1:2,000
1:50-1:100
1:200-1:1,000
Uniprot #: SwissProt: Q9Y230 Human | Q9WTM5 Mouse
Entrez Gene: 292907 Rat
Alternative names: 48 kDa TATA box-binding protein-interacting protein 48 kDa TBP-interacting protein 48-kDa TATA box-binding protein-interacting protein 48-kDa TBP-interacting protein 51 kDa erythrocyte cytosolic protein CGI-46 EC=3.6.1.- ECP-51 ECP51 Erythrocyte cytosolic protein, 51-KD INO80 complex subunit J INO80J MGC144733 MGC144734 MGC52995 mp47 p47 p47 protein Repressing pontin 52 Reptin 52 REPTIN RuvB (E coli homolog)-like 2 RUVB, E. coli, homolog-like 2 RuvB-like 2 (E. coli) RuvB-like 2 RuvB-like protein 2 RUVB2 RUVB2_HUMAN RUVBL2 RVB2 TAP54-beta TATA box-binding protein-interacting protein, 48-KD TBP-interacting protein, 48-KD TIH2 TIP48 TIP49b TIP60-associated protein 54-beta wu:fi25f01 zreptin
Images
EM1901-58_1.jpg Fig1: Western blot analysis of RUVB2 on different lysates with Mouse anti-RUVB2 antibody (EM1901-58) at 1/2,000 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: Daudi cell lysate (20 µg/Lane)
Lane 3: HepG2 cell lysate (20 µg/Lane)
Lane 4: SK-Br-3 cell lysate (20 µg/Lane)
Lane 5: 293T cell lysate (20 µg/Lane)
Lane 6: NIH/3T3 cell lysate (20 µg/Lane)
Lane 7: RAW264.7 cell lysate (20 µg/Lane)
Lane 8: C6 cell lysate (20 µg/Lane)
Lane 9: PC-12 cell lysate (20 µg/Lane)
Lane 10: COS-1 cell lysate (20 µg/Lane)
Lane 11: Mouse testis tissue lysate (40 µg/Lane)
Lane 12: Rat testis tissue lysate (40 µg/Lane)
Lane 13: Mouse thymus tissue lysate (40 µg/Lane)
Lane 14: Rat thymus tissue lysate (40 µg/Lane)

Predicted band size: 51 kDa
Observed band size: 51 kDa

Exposure time: 24 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-58) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
EM1901-58_2.jpg Fig2: Immunocytochemistry analysis of HeLa cells labeling RUVB2 with Mouse anti-RUVB2 antibody (EM1901-58) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-RUVB2 antibody (EM1901-58) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.
EM1901-58_3.jpg Fig3: Immunocytochemistry analysis of RAW264.7 cells labeling RUVB2 with Mouse anti-RUVB2 antibody (EM1901-58) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-RUVB2 antibody (EM1901-58) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.
EM1901-58_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human trachea tissue with Mouse anti-RUVB2 antibody (EM1901-58) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-58) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-58_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue with Mouse anti-RUVB2 antibody (EM1901-58) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-58) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-58_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human testis tissue with Mouse anti-RUVB2 antibody (EM1901-58) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-58) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-58_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Mouse anti-RUVB2 antibody (EM1901-58) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-58) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-58_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded rat testis tissue with Mouse anti-RUVB2 antibody (EM1901-58) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-58) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.