EM1901-59

RUVB2 Mouse Monoclonal Antibody [A1F1]

cat.: EM1901-59

Product Type:	Mouse monoclonal IgG2b, primary antibodies
Species reactivity:	Human, Rat, Mouse
Applications:	WB, IF-Cell, IHC-P, FC
Clonality:	Monoclonal
Clone number:	A1F1

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	2ug/ul
Purification:	Protein G affinity purified.
Molecular weight:	Predicted band size: 51 kDa
Isotype:	IgG2b
Immunogen:	Recombinant protein within Human RUVB2 aa 130-321 / 463.
Positive control:	A549 cell lysates, A549, HT-29, rat trachea tissue, human trachea tissue, human placenta tissue, human testis tissue, HL-60.
Subcellular location:	Nucleus matrix, nucleoplasm, cytoplasm, membrane.
Recommended Dilutions: WB IF-Cell IHC-P FC	1:500-1:2,000 1:50-1:100 1:200-1:1,000 1:50-1:100
Uniprot #:	SwissProt: Q9Y230 Human \| Q9WTM5 Mouse
Alternative names:	48 kDa TATA box-binding protein-interacting protein 48 kDa TBP-interacting protein 48-kDa TATA box-binding protein-interacting protein 48-kDa TBP-interacting protein 51 kDa erythrocyte cytosolic protein CGI-46 EC=3.6.1.- ECP-51 ECP51 Erythrocyte cytosolic protein, 51-KD INO80 complex subunit J INO80J MGC144733 MGC144734 MGC52995 mp47 p47 p47 protein Repressing pontin 52 Reptin 52 REPTIN RuvB (E coli homolog)-like 2 RUVB, E. coli, homolog-like 2 RuvB-like 2 (E. coli) RuvB-like 2 RuvB-like protein 2 RUVB2 RUVB2_HUMAN RUVBL2 RVB2 TAP54-beta TATA box-binding protein-interacting protein, 48-KD TBP-interacting protein, 48-KD TIH2 TIP48 TIP49b TIP60-associated protein 54-beta wu:fi25f01 zreptin

Images

	Fig1: Western blot analysis of RUVB2 on A549 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-59, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
	Fig2: ICC staining of RUVB2 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1901-59, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
	Fig3: ICC staining of RUVB2 in HT-29 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1901-59, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
	Fig4: Immunohistochemical analysis of paraffin-embedded rat trachea tissue tissue using anti-RUVB2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (EM1901-59, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig5: Immunohistochemical analysis of paraffin-embedded human trachea tissue with Mouse anti-RUVB2 antibody (EM1901-59) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (EM1901-59) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig6: Immunohistochemical analysis of paraffin-embedded human placenta tissue with Mouse anti-RUVB2 antibody (EM1901-59) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (EM1901-59) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig7: Immunohistochemical analysis of paraffin-embedded human testis tissue with Mouse anti-RUVB2 antibody (EM1901-59) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (EM1901-59) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig8: Flow cytometric analysis of RUVB2 was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-59, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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