LYRIC Mouse Monoclonal Antibody [C6-C10]
cat.: EM1901-60
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | C6-C10 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size 64 kDa. |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human LYRIC aa 6-233 / 582. |
| Positive control: | MCF-7 cell lysate, Jurkat cell lysate, rat testis tissue, human breast carcinoma tissue, mouse brain tissue, K562, Jurkat. |
| Subcellular location: | Nucleus membrane, nucleolus, endoplasmic reticulum membrane, tight junction, perinuclear region. |
| Recommended Dilutions:
WB IHC-P FC IF-Cell |
1:500-1:2,000 1:200-1:1,000 1:50-1:100 1:100 |
| Uniprot #: | SwissProt: Q86UE4 Human | Q80WJ7 Mouse | Q9Z1W6 Rat |
| Alternative names: | 3D3 3D3/LYRIC AEG 1 AEG-1 AEG1 Astrocyte elevated gene 1 Astrocyte elevated gene-1 protein LYRIC LYRIC/3D3 LYRIC_HUMAN Lysine rich CEACAM1 associated protein Lysine rich CEACAM1 co isolated protein Lysine-rich CEACAM1 co-isolated protein Metadherin Metastasis adhesion protein MTDH Protein LYRIC |
Images
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Fig1:
Western blot analysis of LYRIC on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-60, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell lysate Lane 2: Jurkat cell lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-LYRIC antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-60, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-LYRIC antibody (EM1901-60) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-60) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-LYRIC antibody (EM1901-60) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-60) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Flow cytometric analysis of LYRIC was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-60, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig6:
Immunocytochemistry analysis of Jurkat cells labeling LYRIC with Mouse anti-LYRIC antibody (EM1901-60) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-LYRIC antibody (EM1901-60) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.