LYRIC Mouse Monoclonal Antibody [6-F4]
cat.: EM1901-61
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | 6-F4 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 64 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human LYRIC aa 6-233 / 582. |
| Positive control: | Jurkat cell lysate, Hela cell lysate, K562. |
| Subcellular location: | Nucleus membrane, nucleolus, endoplasmic reticulum membrane, tight junction, perinuclear region, cytoplasm. |
| Recommended Dilutions:
WB FC |
1:500-1:2,000 1:50-1:100 |
| Uniprot #: | SwissProt: Q86UE4 Human |
| Alternative names: | 3D3 3D3/LYRIC AEG 1 AEG-1 AEG1 Astrocyte elevated gene 1 Astrocyte elevated gene-1 protein LYRIC LYRIC/3D3 LYRIC_HUMAN Lysine rich CEACAM1 associated protein Lysine rich CEACAM1 co isolated protein Lysine-rich CEACAM1 co-isolated protein Metadherin Metastasis adhesion protein MTDH Protein LYRIC |
Images
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Fig1:
Western blot analysis of LYRIC on different lysates with Rabbit anti-LYRIC antibody (EM1901-61) at 1/5,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: Jurkat (Human T-lymphoblastic cells) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 180 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: EM1901-61, 1/20,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 64 kDa Observed band size: 80 kDa |
|
Fig2: Flow cytometric analysis of LYRIC was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-61, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.