CLEC4F Mouse Monoclonal Antibody [10-A6-1]
cat.: EM1901-63
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human
Applications: WB, FC
Clonality: Monoclonal
Clone number: 10-A6-1
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: Predicted band size 65 kDa.
Isotype: IgG1
Immunogen: Recombinant protein within Human CLEC4F aa 93-426 / 589.
Positive control: SH-SY5Y cell lysates, SH-SY5Y.
Subcellular location: Membrane.
Recommended Dilutions:
  WB
  FC

1:500-1:2,000
1:50-1:100
Uniprot #: SwissProt: Q8N1N0 Human
Alternative names: C type (calcium dependent, carbohydrate recognition domain) lectin, superfamily member 13 C-type lectin 13 C-type lectin domain family 4 member F C-type lectin superfamily member 13 CLC4F_HUMAN Clec4f CLECSF13 KCLR
Images
EM1901-63_1.jpg Fig1: Western blot analysis of CLEC4F on SH-SY5Y cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-63, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
EM1901-63_2.jpg Fig2: Flow cytometric analysis of CLEC4F was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-63, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.