CLEC4F Mouse Monoclonal Antibody [10-A6-1]
cat.: EM1901-63
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | 10-A6-1 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 66 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human CLEC4F aa 93-426 / 589. |
| Positive control: | SH-SY5Y cell lysates, SH-SY5Y. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB FC |
1:500-1:2,000 1:50-1:100 |
| Uniprot #: | SwissProt: Q8N1N0 Human |
| Alternative names: | C type (calcium dependent, carbohydrate recognition domain) lectin, superfamily member 13 C-type lectin 13 C-type lectin domain family 4 member F C-type lectin superfamily member 13 CLC4F_HUMAN Clec4f CLECSF13 KCLR |
Images
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Fig1: Western blot analysis of CLEC4F on SH-SY5Y cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-63, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Flow cytometric analysis of CLEC4F was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-63, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.