UBA3 Mouse Monoclonal Antibody [1B6-6-5]
cat.: EM1901-64
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | 1B6-6-5 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 52 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human UBA3 aa 220-424 / 463. |
| Positive control: | 293T cell lysates, 293 cell lysate, HL-60 cell lysate, human lung tissue, human lung carcinoma tissue, human breast carcinoma tissue, SiHa. |
| Subcellular location: | Cytoplasm, cytosol, nucleus. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:2,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q8TBC4 Human |
| Alternative names: | DKFZp566J164 EC 6.3.2. hUba3 MGC22384 NEDD8 activating enzyme E1 catalytic subunit NEDD8 activating enzyme E1C Nedd8 activating enzyme hUba3 NEDD8-activating enzyme E1 catalytic subunit NEDD8-activating enzyme E1C uba3 UBA3 ubiquitin activating enzyme E1 homolog UBA3_HUMAN UBE1C Ubiquitin activating enzyme 3 Ubiquitin activating enzyme E1C Ubiquitin-activating enzyme 3 Ubiquitin-activating enzyme E1C Ubiquitin-like modifier-activating enzyme 3 |
Images
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Fig1:
Western blot analysis of UBA3 on different lysates with Rabbit anti-UBA3 antibody (EM1901-64) at 1/5,000 dilution. Lane 1: HEK-293 (Human embryonic kidney cell) Lysates/proteins at 10 µg/Lane. Exposure time: 2 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: EM1901-64, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 52 kDa Observed band size: 52 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-UBA3 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-64, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-UBA3 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-64, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-UBA3 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-64, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Flow cytometric analysis of UBA3 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-64, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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