CEACAM6 Mouse Monoclonal Antibody [A1E2]
cat.: EM1901-68
| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | A1E2 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 37 kDa |
| Isotype: | IgG2b |
| Immunogen: | Recombinant protein within Human CEACAM6 aa 1-160 / 344. |
| Positive control: | SK-Br-3 cell lysate, A549 cell lysate, human colon tissue, human colon cancer tissue, A549, HT-29, MCF-7, SW620. |
| Subcellular location: | Cell membrane, apical cell membrane, cell surface. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:1,000-1:2,000 1:50-1:100 1:50-1:400 1:50-1:100 |
| Uniprot #: | SwissProt: P40199 Human |
| Alternative names: | Carcinoembryonic antigen related cell adhesion molecule 6 Carcinoembryonic antigen related cell adhesion molecule 6 (non specific cross reacting antigen) Carcinoembryonic antigen-related cell adhesion molecule 6 CD 66c CD66c CD66c antigen CEA LIKE PROTEIN CEACAM 6 CEACAM6 CEAL CEAM6_HUMAN MGC93832 NCA Non specific cross reacting antigen Non-specific crossreacting antigen Normal cross reacting antigen Normal cross-reacting antigen |
Images
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Fig1:
Western blot analysis of CEACAM6 on different lysates with Mouse anti-CEACAM6 antibody (EM1901-68) at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: A549 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 37 kDa Observed band size: 37-250 kDa Exposure time: 11 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-68) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Mouse anti-CEACAM6 antibody (EM1901-68) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-68) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-CEACAM6 antibody (EM1901-68) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-68) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: ICC staining of CEACAM6 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1901-68, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig5: ICC staining of CEACAM6 in HT-29 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1901-68, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig6: ICC staining of CEACAM6 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1901-68, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig7: Flow cytometric analysis of CEACAM6 was done on SW620 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-68, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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