CEACAM6 Mouse Monoclonal Antibody [A1E3]
cat.: EM1901-69
Product Type: Mouse monoclonal IgG2a, primary antibodies
Species reactivity: Human
Applications: ICC, IHC-P, FC
Clonality: Monoclonal
Clone number: A1E3
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: Predicted band size 37 kDa.
Isotype: IgG2a
Immunogen: Recombinant protein within human CEACAM6 aa 1-150.
Positive control: HT-29, human lung tissue, human colon tissue, human colon carcinoma tissue, SW620.
Subcellular location: Cell membrane, apical cell membrane, cell surface.
Recommended Dilutions:
  ICC
  IHC-P
  FC

1:50-1:100
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P40199 Human
Alternative names: Carcinoembryonic antigen related cell adhesion molecule 6 Carcinoembryonic antigen related cell adhesion molecule 6 (non specific cross reacting antigen) Carcinoembryonic antigen-related cell adhesion molecule 6 CD 66c CD66c CD66c antigen CEA LIKE PROTEIN CEACAM 6 CEACAM6 CEAL CEAM6_HUMAN MGC93832 NCA Non specific cross reacting antigen Non-specific crossreacting antigen Normal cross reacting antigen Normal cross-reacting antigen
Images
EM1901-69_1.jpg Fig1: ICC staining of CEACAM6 in HT-29 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1901-69, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
EM1901-69_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-CEACAM6 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-69, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-69_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-CEACAM6 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-69, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-69_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-CEACAM6 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-69, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-69_5.jpg Fig5: Flow cytometric analysis of CEACAM6 was done on SW620 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-69, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.