CD43 Mouse Monoclonal Antibody [A2F8]
cat.: EM1901-70
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | A2F8 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 40 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within C-terminal human CD43. |
| Positive control: | K562 cell lysates, human B lymphocyte tumor tissue, human tonsil tissue, HL-60. |
| Subcellular location: | Membrane, microvillus, uropodium, Nucleus, PML body. |
| Recommended Dilutions:
WB IHC-P FC |
1:500 1:1,000 1:50-1:100 |
| Uniprot #: | SwissProt: P16150 Human |
| Alternative names: | CD 43 CD43 CD43 antigen Galactoglycoprotein GALGP GPL 115 GPL115 Human gene for sialophorin Leucocyte sialoglycoprotein LEUK_HUMAN Leukocyte large sialoglycoprotein Leukocyte sialoglycoprotein Leukosialin LSN Ly-48 sialophorin (gpL115, leukosialin, CD43) Sialophorin Spn |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human B lymphocyte tumor tissue with Mouse anti-CD43 antibody (EM1901-70) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-70) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-CD43 antibody (EM1901-70) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-70) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Flow cytometric analysis of CD43 was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-70, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig4:
Western blot analysis of CD43 on different lysates with Rabbit anti-CD43 antibody (EM1901-70) at 1/1,000 dilution. Lane 1: HL-60 (Human acute promyelocytic leukemia cells) cell lysat Lane 2: THP-1 (Human acute monoblastic leukemia cell) cell lysate Lane 3: U-937 (Human acute monocytic leukemia cells) cell lysate Lane 4: HT-29 (Human colorectal adenocarcinoma cell) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 25 seconds; ECL: K1802 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: EM1901-70, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 40 kDa Observed band size: 100-130 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.