CD43 Mouse Monoclonal Antibody [A2F9]
cat.: EM1901-71
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: A2F9
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/mL.
Purification: Protein G affinity purified.
Molecular weight: Predicted band size: 40 kDa
Isotype: IgG1
Immunogen: Synthetic peptide within C-terminal human CD43.
Positive control: K562 cell lysates, human tonsil tissue, HL-60.
Subcellular location: Membrane, microvillus, uropodium, Nucleus, PML body.
Recommended Dilutions:
  WB
  IHC-P
  FC

1:500
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P16150 Human
Alternative names: CD 43 CD43 CD43 antigen Galactoglycoprotein GALGP GPL 115 GPL115 Human gene for sialophorin Leucocyte sialoglycoprotein LEUK_HUMAN Leukocyte large sialoglycoprotein Leukocyte sialoglycoprotein Leukosialin LSN Ly-48 sialophorin (gpL115, leukosialin, CD43) Sialophorin Spn
Images
EM1901-71_1.jpg Fig1: Western blot analysis of CD43 on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-71, 1/200) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
EM1901-71_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD43 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-71, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-71_3.jpg Fig3: Flow cytometric analysis of CD43 was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-71, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.