Annexin A3 Mouse Monoclonal Antibody [A1F7]
cat.: EM1901-73
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Monoclonal
Clone number: A1F7
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein G affinity purified.
Molecular weight: Predicted band size: 36 kDa
Isotype: IgG1
Immunogen: Recombinant protein within Human Annexin A3 aa 1-323 / 323.
Positive control: A431 cell lysate, HEK-293 cell lysate, HepG2 cell lysate, HeLa cell lysate, HT-29 cell lysate, A375 cell lysate, MCF7 cell lysate, human lung tissue lysate, mouse lung tissue lysate, mouse testis tissue lysate, rat testis tissue lysate, HT-29, SiHa, human tonsil tissue, human kidney tissue, human pancreas tissue, mouse lung tissue, A431.
Subcellular location: Cytosol, extracellular exosome, plasma membrane, axon, cytoplasm, dendrite, membrane, neuronal cell body, phagocytic vesicle membrane, specific granule.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:2,000
1:50-1:100
1:50-1:500
1:50-1:100
Uniprot #: SwissProt: P12429 Human | O35639 Mouse | P14669 Rat
Alternative names: 2-cyclic phosphate 2-phosphohydrolase 35-alpha calcimedin Annexin A3 Annexin III Annexin-3 ANX3 ANXA 3 Anxa3 ANXA3_HUMAN Calcimedin 35 alpha Inositol 1 2 cyclic phosphate 2 phosphohydrolase Inositol 1 Lipocortin III OTTHUMP00000160701 OTTHUMP00000219237 OTTHUMP00000219238 PAP III PAP-III Placental anticoagulant protein III
Images
EM1901-73_1.jpg Fig1: Western blot analysis of Annexin A3 on different lysates with Mouse anti-Annexin A3 antibody (EM1901-73) at 1/2,000 dilution.

Lane 1: A431 cell lysate (20 µg/Lane)
Lane 2: HEK-293 cell lysate (20 µg/Lane)
Lane 3: HepG2 cell lysate (20 µg/Lane)
Lane 4: HeLa cell lysate (20 µg/Lane)
Lane 5: HT-29 cell lysate (20 µg/Lane)
Lane 6: A375 cell lysate (20 µg/Lane)
Lane 7: MCF7 cell lysate (20 µg/Lane)
Lane 8: Human lung tissue lysate (40 µg/Lane)
Lane 9: Mouse lung tissue lysate (40 µg/Lane)
Lane 10: Mouse testis tissue lysate (40 µg/Lane)
Lane 11: Rat testis tissue lysate (40 µg/Lane)

Predicted band size: 36 kDa
Observed band size: 33/36 kDa

Exposure time: 9 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-73) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
EM1901-73_2.jpg Fig2: ICC staining of Annexin A3 in HT-29 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1901-73, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
EM1901-73_3.jpg Fig3: ICC staining of Annexin A3 in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1901-73, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
EM1901-73_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Annexin A3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-73, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-73_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Annexin A3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-73, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-73_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Annexin A3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-73, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-73_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Annexin A3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-73, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-73_8.jpg Fig8: Flow cytometric analysis of Annexin A3 was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-73, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.