Anti-Annexin A3 antibody [A1F7]
cat.: EM1901-73
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, ICC, IHC-P, FC
Clonality: Monoclonal
Clone number: A1F7
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/mL.
Purification: Protein G purified.
Molecular weight: 36 kDa
Isotype: IgG1
Immunogen: Recombinant full length protein of human Annexin A3.
Positive control: MCF-7 cell lysates, HT-29, SiHa, human tonsil tissue, human kidney tissue, human pancreas tissue, A431.
Subcellular location: Cytosol, extracellular exosome, plasma membrane, axon, cytoplasm, dendrite, membrane, neuronal cell body, phagocytic vesicle membrane, specific granule.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:500-1:2,000
1:50-1:100
1:50-1:500
1:50-1:100
Uniprot #: SwissProt: P12429 Human
Alternative names: 2-cyclic phosphate 2-phosphohydrolase antibody 35-alpha calcimedin antibody Annexin A3 antibody Annexin III antibody Annexin-3 antibody ANX3 antibody ANXA 3 antibody Anxa3 antibody ANXA3_HUMAN antibody Calcimedin 35 alpha antibody Inositol 1 2 cyclic phosphate 2 phosphohydrolase antibody Inositol 1 antibody Lipocortin III antibody OTTHUMP00000160701 antibody OTTHUMP00000219237 antibody OTTHUMP00000219238 antibody PAP III antibody PAP-III antibody Placental anticoagulant protein III antibody
Images
EM1901-73_1.jpg Fig1: Western blot analysis of Annexin A3 on MCF-7 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-73, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
EM1901-73_2.jpg Fig2: ICC staining of Annexin A3 in HT-29 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1901-73, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
EM1901-73_3.jpg Fig3: ICC staining of Annexin A3 in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1901-73, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
EM1901-73_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Annexin A3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-73, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-73_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Annexin A3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-73, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-73_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Annexin A3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-73, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-73_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Annexin A3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-73, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-73_8.jpg Fig8: Flow cytometric analysis of Annexin A3 was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-73, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.