MTA2 Mouse Monoclonal Antibody [17A2]
cat.: EM1901-74
Product Type: Mouse monoclonal IgG2b, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: 17A2
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/mL.
Purification: Protein G affinity purified.
Molecular weight: 75 kDa
Isotype: IgG2b
Immunogen: Recombinant protein within Human MTA2 aa 77-286 / 668.
Positive control: K562 cell lysate, MCF-7 cell lysate, SH-SY5Y cell lysate, Daudi cell lysate, human tonsil tissue, human colon carcinoma tissue, human skin tissue, human breast tissue, human breast carcinoma tissue, human esophagus tissue, human pancreas tissue, MCF-7.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IHC-P
  FC

1:500-1:2,000
1:500-1:1,000
1:50-1:100
Uniprot #: SwissProt: O94776 Human
Alternative names: DKFZp686F2281 Mata1l1 Metastasis associated 1 family member 2 Metastasis associated 1 like 1 Metastasis associated gene 1 like 1 Metastasis associated gene family member 2 Metastasis associated protein 2 Metastasis associated protein MTA 2 Metastasis associated protein MTA2 Metastasis-associated 1-like 1 Metastasis-associated protein MTA2 Mmta2 MTA1 L1 protein MTA1-L1 protein MTA1L1 MTA2 MTA2_HUMAN p53 target protein in deacetylase complex PID
Images
EM1901-74_1.jpg Fig1: Western blot analysis of MTA2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-74, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: K562 cell lysate
Lane 2: MCF-7 cell lysate
Lane 3: SH-SY5Y cell lysate
Lane 4: Daudi cell lysate
EM1901-74_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MTA2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-74, 1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-74_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-MTA2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-74, 1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-74_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-MTA2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-74, 1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-74_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-MTA2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-74, 1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-74_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-MTA2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-74, 1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-74_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-MTA2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-74, 1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-74_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-MTA2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-74, 1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-74_9.jpg Fig9: Flow cytometric analysis of MTA2 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-74, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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