MTA2 Mouse Monoclonal Antibody [17A2]
cat.: EM1901-74
Product Type: Mouse monoclonal IgG2b, primary antibodies
Species reactivity: Human, Rat, Mouse
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: 17A2
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein G affinity purified.
Molecular weight: Predicted band size: 75 kDa
Isotype: IgG2b
Immunogen: Recombinant protein within Human MTA2 aa 77-286 / 668.
Positive control: HeLa cell lysate, K-562 cell lysate, 293T cell lysate, Jurkat cell lysate, Raji cell lysate, MCF7 cell lysate, human breast cancer tissue, human colon cancer tissue, human skin tissue, rat skin tissue, human breast tissue, human esophagus tissue, human pancreas tissue, MCF-7
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IHC-P
  FC
1:500-1:3,000
1:500-1:3,000
1:50-1:100
Uniprot #: SwissProt: O94776 Human | Q9R190 Mouse
Entrez Gene: 361724 Rat
Alternative names: DKFZp686F2281 Mata1l1 Metastasis associated 1 family member 2 Metastasis associated 1 like 1 Metastasis associated gene 1 like 1 Metastasis associated gene family member 2 Metastasis associated protein 2 Metastasis associated protein MTA 2 Metastasis associated protein MTA2 Metastasis-associated 1-like 1 Metastasis-associated protein MTA2 Mmta2 MTA1 L1 protein MTA1-L1 protein MTA1L1 MTA2 MTA2_HUMAN p53 target protein in deacetylase complex PID
Images
EM1901-74_1.jpg Fig1: Western blot analysis of MTA2 on different lysates with Mouse anti-MTA2 antibody (EM1901-74) at 1/3,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: K-562 cell lysate
Lane 3: 293T cell lysate
Lane 4: Jurkat cell lysate
Lane 5: Raji cell lysate
Lane 6: MCF7 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 75 kDa
Observed band size: 75 kDa

Exposure time: 3 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-74) at 1/3,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
EM1901-74_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Mouse anti-MTA2 antibody (EM1901-74) at 1/3,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-74) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-74_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-MTA2 antibody (EM1901-74) at 1/3,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-74) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-74_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human skin tissue with Mouse anti-MTA2 antibody (EM1901-74) at 1/600 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-74) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-74_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat skin tissue with Mouse anti-MTA2 antibody (EM1901-74) at 1/600 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-74) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-74_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-MTA2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-74, 1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-74_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-MTA2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-74, 1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-74_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-MTA2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-74, 1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-74_9.jpg Fig9: Flow cytometric analysis of MTA2 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-74, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.