Cytokeratin 19 Mouse Monoclonal Antibody [A3D5]
cat.: EM1901-76
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P, IF-Cell, FC
Clonality: Monoclonal
Clone number: A3D5
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 44 kDa
Isotype: IgG1
Immunogen: Recombinant protein within Human Keratin 19 aa 128-322.
Positive control: MCF-7 cell lysates, JAR, human pancreas tissue, human colon cancer tissue, human breast tissue, human small intestine tissue, MCF-7.
Subcellular location: Cytoskeleton,Cytosol.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
  FC
1:1,000-1:5,000
1:500-1:2,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P08727 Human
Alternative names: 40 kDa keratin intermediate filament CK 19 CK-19 CK19 Cytokeratin 19 Cytokeratin-19 K19 K1C19_HUMAN K1CS Keratin 19 Keratin type I 40 kD Keratin type I 40kD Keratin type I cytoskeletal 19 Keratin, type I cytoskeletal 19 Keratin, type I, 40 kd Keratin-19 KRT19 MGC15366
Images
EM1901-76_1.jpg Fig1: Western blot analysis of Cytokeratin 19 on different lysates with Mouse anti-Cytokeratin 19 antibody (EM1901-76) at 1/1,000 dilution.

Lane 1: HCT 116-si NT cell lysate
Lane 2: HCT 116-si Cytokeratin 19 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 44 kDa
Observed band size: 40/44 kDa

Exposure time: 20 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-76) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
EM1901-76_2.jpg Fig2: Western blot analysis of Cytokeratin 19 on MCF-7 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-76, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
EM1901-76_3.jpg Fig3: ICC staining of Cytokeratin 19 in JAR cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1901-76, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
EM1901-76_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Cytokeratin 19 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-76, 1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-76_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Cytokeratin 19 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-76, 1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-76_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Cytokeratin 19 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-76, 1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-76_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-Cytokeratin 19 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-76, 1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-76_8.jpg Fig8: Flow cytometric analysis of Cytokeratin 19 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-76, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488 Goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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