NF-kB p100 / NFKB2 Mouse Monoclonal Antibody [16B2]
cat.: EM1901-78
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | 16B2 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 97 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within Human NFKB2 aa 1-50 / 900. |
| Positive control: | Human tonsil tissue, human placenta tissue, A431 cell, Siha cell lysates. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:2000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q00653 Human |
| Alternative names: | CVID10 DNA binding factor KBF2 H2TF1 Lymphocyte translocation chromosome 10 protein LYT 10 NF kB2 NFKB p52/p100 subunit Nuclear factor Kappa B subunit 2 Nuclear factor of kappa light polypeptide gene enhancer in B cells 2 (p49/p100) Nuclear factor of kappa light polypeptide gene enhancer in B cells 2 Oncogene Lyt 10 p100 Transcription factor NFKB2 |
Images
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Fig1:
Western blot analysis of NF-kB p100 / NFKB2 on Siha cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-78, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. Predicted band size: 97 kDa Observed band size: 52/100 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NF-kB p100 / NFKB2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-78, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-NF-kB p100 / NFKB2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-78, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Flow cytometric analysis of NF-kB p100 / NFKB2 was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-78, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488 Goat anti-Mouse IgG antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.