Anti-NFKB2 antibody [16B2]
cat.: EM1901-78
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: 16B2
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 52/97 kDa
Isotype: IgG1
Immunogen: Synthetic peptide corresponding to N terminal Human NFKB2.
Positive control: Human tonsil tissue, human placenta tissue, A431 cell, Siha cell lysates.
Subcellular location: Nucleus, Cytoplasm.
Recommended Dilutions:
  WB
  IHC-P
  FC

1:500-1:2000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q00653 Human
Alternative names: CVID10 DNA binding factor KBF2 H2TF1 Lymphocyte translocation chromosome 10 protein LYT 10 NF kB2 NFKB p52/p100 subunit Nuclear factor Kappa B subunit 2 Nuclear factor of kappa light polypeptide gene enhancer in B cells 2 (p49/p100) Nuclear factor of kappa light polypeptide gene enhancer in B cells 2 Oncogene Lyt 10 p100 Transcription factor NFKB2
Images
EM1901-78_1.jpg Fig1: Western blot analysis of NFKB2 on Siha cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-78, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Predicted band size: 97 kDa
Observed band size: 52/100 kDa
EM1901-78_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NFKB2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-78, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-78_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-NFKB2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-78, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-78_4.jpg Fig4: Flow cytometric analysis of NFKB2 was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-78, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa FluorTM488 Goat anti-Mouse IgG antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.