Albumin Mouse Monoclonal Antibody [1-G10]
cat.: EM1901-81
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC, ELISA |
| Clonality: | Monoclonal |
| Clone number: | 1-G10 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 69 kDa |
| Isotype: | IgG1 |
| Immunogen: | Native protein. |
| Positive control: | Human plasma tissue lysates, HepG2. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB FC ELISA |
1:10,000-1:50,000 1:50-1:100 1: 5,000-1:20,000 |
| Uniprot #: | SwissProt: P02768 Human |
| Alternative names: | alb ALBU_HUMAN Albumin (32 AA) Albumin (AA 34) Albumin Analbuminemia Bisalbuminemia Cell growth inhibiting protein 42 DKFZp779N1935 Dysalbuminemic hyperthyroxinemia Growth inhibiting protein 20 HSA Hyperthyroxinemia dysalbuminemic PRO0883 PRO0903 PRO1341 Serum albumin |
Images
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Fig1: Western blot analysis of Albumin on human plasma sample. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-81, 1/50000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig2: Flow cytometric analysis of Albumin was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-81, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with Alexa FluorTM488 Goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig3:
Albumin Antibody (EM1901-81) in indirect ELISA. Indirect ELISA analysis of Albumin was performed by coating wells of a 96-well plate with 50 µl per well of human albumin antigen or BSA diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with StartingBlock blocking buffer, and incubated with 50 µl per well of a mouse Albumin monoclonal antibody starting at a concentration of 2 µg/mL and serially diluting it to a concentration of 1.95 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.