CAPZA1 Mouse Monoclonal Antibody [A2E7]
cat.: EM1901-82
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human
Applications: WB, IF-Cell, IHC-P
Clonality: Monoclonal
Clone number: A2E7
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein G affinity purified.
Molecular weight: Predicted band size: 33 kDa
Isotype: IgG1
Immunogen: Recombinant protein within Human CAPZA1 aa 60-248 / 286.
Positive control: K-562 cell lysate, 293T cell lysate, Jurkat cell lysate, HeLa cell lysate, HepG2 cell lysate, A549 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C2C12 cell lysate, PC-12 cell lysate, HeLa, human prostate carcinoma tissue, human kidney tissue, human colon cancer tissue.
Subcellular location: Cytoskeleton.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
1:5,000
1:1:100
1:50-1:1,000
Uniprot #: SwissProt: P52907 Human | P47753 Mouse | B2GUZ5 Rat
Alternative names: Cap Z Cappa 1 Cappa1 Capping protein (actin filament) muscle Z line alpha 1 Capping protein alpha 1 Capping protein muscle Z line alpha 1 CapZ alpha 1 CapZ alpha-1 CAPZ CAPZA 1 Capza1 CAZ 1 CAZ1 CAZA1_HUMAN F actin capping protein alpha 1 subunit F-actin-capping protein subunit alpha-1
Images
EM1901-82_1.jpg Fig1: Western blot analysis of CAPZA1 on different lysates with Mouse anti-CAPZA1 antibody (EM1901-82) at 1/5,000 dilution.

Lane 1: K-562 cell lysate
Lane 2: 293T cell lysate
Lane 3: Jurkat cell lysate
Lane 4: HeLa cell lysate
Lane 5: HepG2 cell lysate
Lane 6: A549 cell lysate
Lane 7: NIH/3T3 cell lysate
Lane 8: RAW264.7 cell lysate
Lane 9: C2C12 cell lysate
Lane 10: PC-12 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 33 kDa
Observed band size: 33 kDa

Exposure time: 46 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-82) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
EM1901-82_2.jpg Fig2: Immunocytochemistry analysis of HeLa cells labeling CAPZA1 with Mouse anti-CAPZA1 antibody (EM1901-82) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CAPZA1 antibody (EM1901-82) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.
EM1901-82_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue using anti-CAPZA1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-82, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-82_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CAPZA1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-82, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-82_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-CAPZA1 antibody (EM1901-82) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-82) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.