CAPZA1 Mouse Monoclonal Antibody [A2E7]
cat.: EM1901-82
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A2E7 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 33 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human CAPZA1 aa 60-248 / 286. |
| Positive control: | K-562 cell lysate, 293T cell lysate, Jurkat cell lysate, HeLa cell lysate, HepG2 cell lysate, A549 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C2C12 cell lysate, PC-12 cell lysate, HeLa, human colon cancer tissue. |
| Subcellular location: | Cytoskeleton. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:5,000 1:100 1:1,000 |
| Uniprot #: | SwissProt: P52907 Human | P47753 Mouse | B2GUZ5 Rat |
| Alternative names: | Cap Z Cappa 1 Cappa1 Capping protein (actin filament) muscle Z line alpha 1 Capping protein alpha 1 Capping protein muscle Z line alpha 1 CapZ alpha 1 CapZ alpha-1 CAPZ CAPZA 1 Capza1 CAZ 1 CAZ1 CAZA1_HUMAN F actin capping protein alpha 1 subunit F-actin-capping protein subunit alpha-1 |
Images
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Fig1:
Western blot analysis of CAPZA1 on different lysates with Mouse anti-CAPZA1 antibody (EM1901-82) at 1/5,000 dilution. Lane 1: K-562 cell lysate Lane 2: 293T cell lysate Lane 3: Jurkat cell lysate Lane 4: HeLa cell lysate Lane 5: HepG2 cell lysate Lane 6: A549 cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: RAW264.7 cell lysate Lane 9: C2C12 cell lysate Lane 10: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 33 kDa Observed band size: 33 kDa Exposure time: 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-82) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HeLa cells labeling CAPZA1 with Mouse anti-CAPZA1 antibody (EM1901-82) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CAPZA1 antibody (EM1901-82) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-CAPZA1 antibody (EM1901-82) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-82) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.