Anti-CAPZA1 antibody [A2E7]
cat.: EM1901-82
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human
Applications: WB, ICC, IHC-P, FC
Clonality: Monoclonal
Clone number: A2E7
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/mL.
Purification: Protein G affinity purified.
Molecular weight: 33 kDa
Isotype: IgG1
Immunogen: Recombinant protein within Human CAPZA1 aa 60-248 / 286.
Positive control: K562 cell lysates, A431, SiHa, human prostate carcinoma tissue, human kidney tissue, HL-60.
Subcellular location: Cytoskeleton.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:500-1:1,000
1:50-1:100
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P52907 Human
Alternative names: Cap Z Cappa 1 Cappa1 Capping protein (actin filament) muscle Z line alpha 1 Capping protein alpha 1 Capping protein muscle Z line alpha 1 CapZ alpha 1 CapZ alpha-1 CAPZ CAPZA 1 Capza1 CAZ 1 CAZ1 CAZA1_HUMAN F actin capping protein alpha 1 subunit F-actin-capping protein subunit alpha-1
Images
EM1901-82_1.jpg Fig1: Western blot analysis of CAPZA1 on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-82, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
EM1901-82_2.jpg Fig2: ICC staining of CAPZA1 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1901-82, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
EM1901-82_3.jpg Fig3: ICC staining of CAPZA1 in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1901-82, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
EM1901-82_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue using anti-CAPZA1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-82, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-82_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CAPZA1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-82, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-82_6.jpg Fig6: Flow cytometric analysis of CAPZA1 was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-82, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.