DNMT1 Mouse Monoclonal Antibody [A3A9]
cat.: EM1901-84
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | A3A9 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | 183 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within Human Dnmt1 aa 500-600 / 1616. |
| Positive control: | Hela cell lysate, 293 cell lysate,Jurkat cell lysate, human tonsil tissue, JAR. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:5,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P26358 Human |
| Alternative names: | ADCADN AIM CXXC finger protein 9 CXXC-type zinc finger protein 9 CXXC9 DNA (cytosine 5 ) methyltransferase 1 DNA (cytosine-5)-methyltransferase 1 DNA methyltransferase 1 DNA methyltransferase HsaI DNA methyltransferase M.HsaI. DNA MTase DNA MTase HsaI DNMT 1 DNMT Dnmt1 DNMT1_HUMAN Dnmt1o FLJ16293 HSN1E M.HsaI MCMT Met1 MGC104992 mMmul MommeD2 |
Images
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Fig1:
Western blot analysis of DNMT1 on different lysates with Mouse anti-DNMT1 antibody (EM1901-84) at 1/5,000 dilution. Lane 1: 293T (Human embryonic kidney cell) cell lysate Lane 2: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 3: Jurkat (Human T-lymphoblastic cells) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 126 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: EM1901-84, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 183 kDa Observed band size: 183 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-DNMT1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-84, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Flow cytometric analysis of DNMT1 was done on JAR cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-84, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.