MCU Mouse Monoclonal Antibody [A2E11]
cat.: EM1901-85
| Product Type: | Mouse monoclonal IgM, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | A2E11 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size 40 kDa |
| Isotype: | IgM |
| Immunogen: | Recombinant protein within Human MCU aa 51-325 / 351. |
| Positive control: | 293 cell lysates, A549. |
| Subcellular location: | Mitochondrion. |
| Recommended Dilutions:
WB |
1:500-1:1,000 |
| Uniprot #: | SwissProt: Q8NE86 Human | Q3UMR5 Mouse Entrez Gene: 294560 Rat |
| Alternative names: | C109A_HUMAN C10orf42 Calcium uniporter protein mitochondrial Ccdc109a Coiled-coil domain-containing protein 109A HsMCU Mitochondrial Calcium Uniporter |
Images
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Fig1:
Western blot analysis of MCU on different lysates with Mouse anti-MCU antibody (EM1901-85) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HeLa cell lysate Lane 3: HepG2 cell lysate Lane 4: PC-12 cell lysate Lane 5: NIH/3T3 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 40 kDa Observed band size: 36 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-85) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.