ACY1 Mouse Monoclonal Antibody [15G2]
cat.: EM1901-86
Product Type: Mouse monoclonal IgG2a, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: 15G2
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 46 kDa
Isotype: IgG2a
Immunogen: Recombinant protein within Human ACY1 aa 55-256 / 408.
Positive control: K-562 cell lysate, HepG2 cell lysate, PC-3M cell lysate, human liver tissue lysate, human kidney tissue lysate, mouse liver tissue lysate, mouse kidney tissue lysate, rat liver tissue lysate, rat kidney tissue lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue, SHSY5Y.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB
  IHC-P
  FC
1:500-1:1,000
1:2,000
1:50-1:100
Uniprot #: SwissProt: Q03154 Human | Q99JW2 Mouse | Q6AYS7 Rat
Alternative names: ACY 1 ACY-1 Acy1 ACY1_HUMAN ACY1D ACYLASE Acylase I Aminoacylase 1 Aminoacylase-1 EC 3.5.1.14 epididymis secretory protein Li 5 HEL-S-5 N acyl L amino acid amidohydrolase N-acyl-L-amino-acid amidohydrolase OTTHUMP00000212459 OTTHUMP00000212462 OTTHUMP00000212463 OTTHUMP00000212464 OTTHUMP00000212465
Images
EM1901-86_1.jpg Fig1: Western blot analysis of ACY1 on different lysates with Mouse anti-ACY1 antibody (EM1901-86) at 1/1,000 dilution.

Lane 1: K-562 cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Lane 3: PC-3M cell lysate (20 µg/Lane)
Lane 4: Human liver tissue lysate (40 µg/Lane)
Lane 5: Human kidney tissue lysate (40 µg/Lane)
Lane 6: Mouse liver tissue lysate (40 µg/Lane)
Lane 7: Mouse kidney tissue lysate (40 µg/Lane)
Lane 8: Rat liver tissue lysate (40 µg/Lane)
Lane 9: Rat kidney tissue lysate (40 µg/Lane)

Predicted band size: 46 kDa
Observed band size: 42 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-86) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
EM1901-86_2.jpg Fig2: Western blot analysis of ACY1 on different lysates with Mouse anti-ACY1 antibody (EM1901-86) at 1/1,000 dilution.

Lane 1: A549-si NT cell lysate
Lane 2: A549-si ACY1 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 46 kDa
Observed band size: 46 kDa

Exposure time: 3 minutes; ECL: K1802;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-86) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
EM1901-86_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-ACY1 antibody (EM1901-86) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-86) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-86_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Mouse anti-ACY1 antibody (EM1901-86) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-86) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-86_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Mouse anti-ACY1 antibody (EM1901-86) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-86) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-86_6.jpg Fig6: Flow cytometric analysis of ACY1 was done on SHSY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-86, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa FluorTM488 Goat anti-Mouse IgG IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.