Caveolin-1 Mouse Monoclonal Antibody [A3E7]
cat.: EM1901-87
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | A3E7 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | 20 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human Caveolin-1 aa 1-150. |
| Positive control: | SiHa cell lysates, SKOV-3, human liver tissue, human lung tissue, human lung carcinoma tissue, PANC-1. |
| Subcellular location: | Cell membrane, golgi apparatus membrane, trans-golgi network, caveola, membrane raft. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:2,000 1:50-1:100 1:500-1:1,000 1:50-1:100 |
| Uniprot #: | SwissProt: Q03135 Human |
| Alternative names: | BSCL3 CAV CAV1 CAV1_HUMAN caveolae protein, 22 kD caveolin 1 alpha isoform caveolin 1 beta isoform Caveolin 1 caveolae protein 22kDa Caveolin-1 Caveolin1 cell growth-inhibiting protein 32 CGL3 LCCNS MSTP085 OTTHUMP00000025031 PPH3 VIP 21 VIP21 |
Images
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Fig1: Western blot analysis of Caveolin-1 on SiHa cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-87, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining of Caveolin-1 in SKOV-3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1901-87, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Caveolin-1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-87, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Caveolin-1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-87, 1/1000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-Caveolin-1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-87, 1/1000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6: Flow cytometric analysis of Caveolin-1 was done on PANC-1 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-87, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.