CD1A Mouse Monoclonal Antibody [A3F9]
cat.: EM1901-89
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A3F9 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | 37 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human CD1A aa 50-250. |
| Positive control: | K562 cell lysates, human skin tissue. |
| Subcellular location: | Cell membrane, endosome membrane, membrane raft. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: P06126 Human |
| Alternative names: | CD 1a CD1 CD1a CD1A Antigen CD1A antigen, a polypeptide CD1a molecule CD1A_HUMAN cluster of differentiation 1 A cortical thymocyte antigen CD1A differentiation antigen CD1 alpha 3 epidermal dendritic cell marker CD1a FCB 6 FCB6 HTA 1 HTA1 hTa1 thymocyte antigen OTTHUMP00000018907 R 4 R4 T 6 T-cell surface antigen T6/Leu-6 T-cell surface glycoprotein CD1a T6 |
Images
|
Fig1: Western blot analysis of CD1A on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-89, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-CD1A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-89, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.