Anti-CD1A antibody [A3F9]
cat.: EM1901-89
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: A3F9
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/mL.
Purification: Protein G affinity purified.
Molecular weight: 37 kDa
Isotype: IgG1
Immunogen: Recombinant protein within human CD1A aa 50-250.
Positive control: K562 cell lysates, human skin tissue.
Subcellular location: Cell membrane, endosome membrane, membrane raft.
Recommended Dilutions:
  WB
  IHC-P

1:500-1:1,000
1:50-1:200
Uniprot #: SwissProt: P06126 Human
Alternative names: CD 1a antibody CD1 antibody CD1a antibody CD1A Antigen antibody CD1A antigen, a polypeptide antibody CD1a molecule antibody CD1A_HUMAN antibody cluster of differentiation 1 A antibody cortical thymocyte antigen CD1A antibody differentiation antigen CD1 alpha 3 antibody epidermal dendritic cell marker CD1a antibody FCB 6 antibody FCB6 antibody HTA 1 antibody HTA1 antibody hTa1 thymocyte antigen antibody OTTHUMP00000018907 antibody R 4 antibody R4 antibody T 6 antibody T-cell surface antigen T6/Leu-6 antibody T-cell surface glycoprotein CD1a antibody T6 antibody
Images
EM1901-89_1.jpg Fig1: Western blot analysis of CD1A on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-89, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
EM1901-89_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-CD1A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-89, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.