CD163 Mouse Monoclonal Antibody [A3B5]
cat.: EM1901-91
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | A3B5 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 125 kDa. |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human CD163 aa 1-170. |
| Positive control: | Human liver carcinoma tissue, human placenta tissue, human colon tissue, HT-29. |
| Subcellular location: | Secreted, cell membrane. |
| Recommended Dilutions:
IHC-P FC |
1:100-1:500 1:50-1:100 |
| Uniprot #: | SwissProt: Q86VB7 Human |
| Alternative names: | C163A_HUMAN CD 163 CD163 CD163 antigen CD163 molecule Hemoglobin scavenger receptor M130 M130 antigen precursor Macrophage associated antigen MM130 OTTHUMP00000238617 OTTHUMP00000238618 OTTHUMP00000238619 OTTHUMP00000238620 SCARI1 Scavenger receptor cysteine rich type 1 protein M130 sCD163 Soluble CD163 |
Images
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Fig1: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-CD163 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-91, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-CD163 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-91, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-CD163 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-91, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Flow cytometric analysis of CD163 was done on HT-29 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-91, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.