Cytokeratin 20 Mouse Monoclonal Antibody [A3D12]
cat.: EM1901-96
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human
Applications: WB, FC, IHC-P
Clonality: Monoclonal
Clone number: A3D12
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein G affinity purified.
Molecular weight: 48 kDa
Isotype: IgG1
Immunogen: Synthetic peptide within Human Cytokeratin 20 aa 29-78 / 424.
Positive control: Lovo cell lysates, human small intestine tissue, human colon carcinoma tissue, human stomach carcinoma tissue, JAR.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB
  FC
1:500-1:1,000
1:50-1:100
Uniprot #: SwissProt: P35900 Human
Alternative names: CK 20 CK-20 CK20 Cytokeratin-20 Cytokeratin20 K1C20_HUMAN K20 KA20 Keratin 20 keratin 20, type I keratin 21, rat, homolog of Keratin Keratin type I cytoskeletal 20 Keratin-20 Keratin20 KRT 20 KRT 21 KRT20 KRT21 MGC35423 OTTHUMP00000164518 Protein IT type I cytoskeletal 20
Images
EM1901-96_1.jpg Fig1: Western blot analysis of Cytokeratin 20 on Lovo cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-96, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
EM1901-96_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-Cytokeratin 20 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-96, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-96_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Cytokeratin 20 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-96, 1/500) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-96_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti-Cytokeratin 20 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-96, 1/500) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1901-96_5.jpg Fig5: Flow cytometric analysis of Cytokeratin 20 was done on JAR cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-96, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.