Lysozyme Mouse Monoclonal Antibody [A5A8]
cat.: EM1901-98
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A5A8 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 17 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human Lysozyme aa 1-141 / 148. |
| Positive control: | HepG2 cell lysate, HL-60 cell lysate, human spleen tissue, human stomach cancer tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:1,000 |
| Uniprot #: | SwissProt: P61626 Human |
| Alternative names: | 1 4 beta N acetylmuramidase C 1 4-beta-N-acetylmuramidase C EC 3.2.1.17 LYSC_HUMAN Lysosyme Lysozyme (renal amyloidosis) Lysozyme C Lysozyme C precursor LYZ LZM Renal amyloidosis |
Images
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Fig1:
Western blot analysis of Lysozyme on different lysates with Mouse anti-Lysozyme antibody (EM1901-98) at 1/5,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HL-60 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 17 kDa Observed band size: 14 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-98) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-Lysozyme antibody (EM1901-98) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-98) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue with Mouse anti-Lysozyme antibody (EM1901-98) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-98) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.