| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A3H3 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 586 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human MUC5AC aa 600-850. |
| Positive control: | Human stomach tissue, A549. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
IHC-P |
1:1,000 |
| Uniprot #: | SwissProt: P98088 Human |
| Alternative names: | Apomucin Apomucin Major Airway Glycoprotein Gastric mucin LeB Lewis B blood group antigen Major Airway Glycoprotein MUC 5 MUC5 MUC5AC Mucin 5 subtype AC tracheobronchial Mucin 5 Subtypes A And C Mucin 5 subtypes A and C tracheobronchial/gastric Mucin 5, tracheobronchial/gastric Mucin 5AC Mucin 5AC oligomeric mucus/gel forming Mucin 5AC, oligomeric mucus/gel forming pseudogene Mucin TBM Tracheobronchial Mucin (TBM) Tracheobronchial mucin |
|
Fig1:
Application: Immunohistochemistry (IHC-P) Species: Human Tissue: Stomach Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× PBST Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: EM1902-02, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. |