MUC5AC Mouse Monoclonal Antibody [A3H3]
cat.: EM1902-02
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | A3H3 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 586 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human MUC5AC aa 600-850. |
| Positive control: | Human stomach tissue, A549. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
IHC-P FC |
1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: P98088 Human |
| Alternative names: | Apomucin Apomucin Major Airway Glycoprotein Gastric mucin LeB Lewis B blood group antigen Major Airway Glycoprotein MUC 5 MUC5 MUC5AC Mucin 5 subtype AC tracheobronchial Mucin 5 Subtypes A And C Mucin 5 subtypes A and C tracheobronchial/gastric Mucin 5, tracheobronchial/gastric Mucin 5AC Mucin 5AC oligomeric mucus/gel forming Mucin 5AC, oligomeric mucus/gel forming pseudogene Mucin TBM Tracheobronchial Mucin (TBM) Tracheobronchial mucin |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human stomach tissue with Mouse anti-MUC5AC antibody (EM1902-02) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-02) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2: Flow cytometric analysis of MUC5AC was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1902-02, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.