MUC5AC Mouse Monoclonal Antibody [A3H3]
cat.: EM1902-02
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human
Applications: IHC-P
Clonality: Monoclonal
Clone number: A3H3
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein G affinity purified.
Molecular weight: Predicted band size: 586 kDa
Isotype: IgG1
Immunogen: Recombinant protein within Human MUC5AC aa 600-850.
Positive control: Human stomach tissue, A549.
Subcellular location: Secreted.
Recommended Dilutions:
  IHC-P

1:1,000
Uniprot #: SwissProt: P98088 Human
Alternative names: Apomucin Apomucin Major Airway Glycoprotein Gastric mucin LeB Lewis B blood group antigen Major Airway Glycoprotein MUC 5 MUC5 MUC5AC Mucin 5 subtype AC tracheobronchial Mucin 5 Subtypes A And C Mucin 5 subtypes A and C tracheobronchial/gastric Mucin 5, tracheobronchial/gastric Mucin 5AC Mucin 5AC oligomeric mucus/gel forming Mucin 5AC, oligomeric mucus/gel forming pseudogene Mucin TBM Tracheobronchial Mucin (TBM) Tracheobronchial mucin
Images
EM1902-02_1.jpg Fig1: Application: Immunohistochemistry (IHC-P)

Species: Human
Tissue: Stomach
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

Wash buffer: 1× PBST
Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.

Primary antibody: EM1902-02, 1/1,000, 1 hour at room temperature.

Secondary antibody: HA1120, 20 minutes at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.