ddx24 Mouse Monoclonal Antibody [A3B10]
cat.: EM1902-03
| Product Type: | Mouse monoclonal IgG2a, primary antibodies |
|---|---|
| Species reactivity: | Zebrafish |
| Applications: | IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | A3B10 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | 94 kDa |
| Isotype: | IgG2a |
| Immunogen: | Recombinant protein within zebrafish ddx24 aa 562-819 / 832. |
| Positive control: | Zebrafish tissue. |
| Subcellular location: | Nucleolus. |
| Recommended Dilutions:
IHC-P IF-Tissue |
1:100-1:500 1:200 |
| Uniprot #: | SwissProt: A0A0R4IA19 Zebrafish |
| Alternative names: | DEAD (Asp-Glu-Ala-Asp) box helicase 24 ddx24 |
Images
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Fig1:
Immunofluorescence analysis of paraffin-embedded zebrafish tissue labeling ddx24 with Mouse anti-ddx24 antibody (EM1902-03) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (EM1902-03, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig2: Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-ddx24 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-03, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.