CASK Mouse Monoclonal Antibody [A1C7]
cat.: EM1902-08
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | A1C7 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 105 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human CASK aa 311-513 / 926. |
| Positive control: | A549 cell lysate, Neuro-2a cell lysate, C6 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, C6, human prostate cancer tissue, mouse brain tissue, rat large intestine tissue. |
| Subcellular location: | Cell membrane, Cytoplasm, Membrane, Nucleus. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:2,000 1:50-1:200 1:100 |
| Uniprot #: | SwissProt: O14936 Human | O70589 Mouse | Q62915 Rat |
| Alternative names: | CAGH39 Caki Calcium/calmodulin dependent serine protein kinase Calcium/calmodulin dependent serine protein kinase (MAGUK family) Calcium/calmodulin dependent serine protein kinase membrane associated guanylate kinase Calcium/calmodulin-dependent serine protein kinase CAMGUK CAMGUK protein CAMGUK, drosophila, homolog of casK CMG CSKP_HUMAN DXPri1 DXRib1 FGS4 FLJ22219 FLJ31914 hCASK LIN 2 Lin 2 homolog LIN2 Lin2 homolog MICPCH MRXSNA Pals3 Peripheral plasma membrane protein CASK Protein lin-2 homolog TNRC8 Trinucleotide repeat containing 8 Vertebtate LIN2 homolog |
Images
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Fig1:
Western blot analysis of CASK on different lysates with Mouse anti-CASK antibody (EM1902-08) at 1/2,000 dilution. Lane 1: A549-WT cell lysate Lane 2: A549-KD CASK cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 105 kDa Observed band size: 105 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1902-08) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of CASK on different lysates with Mouse anti-CASK antibody (EM1902-08) at 1/2,000 dilution. Lane 1: Neuro-2a cell lysate Lane 2: C6 cell lysate Lane 3: Mouse brain tissue lysate Lane 4: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 105 kDa Observed band size: 105 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1902-08) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunocytochemistry analysis of C6 cells labeling CASK with Mouse anti-CASK antibody (EM1902-08) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CASK antibody (EM1902-08) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-CASK antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-08, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CASK antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-08, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6: Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-CASK antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-08, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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