CASK Mouse Monoclonal Antibody [A1C7]
cat.: EM1902-08
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: A1C7
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 105 kDa
Isotype: IgG1
Immunogen: Recombinant protein within Human CASK aa 311-513 / 926.
Positive control: Siha cell lysate, A549 cell lysate, rat large intestine tissue, human prostate cancer tissue, mouse brain tissue, SW620.
Subcellular location: Cell membrane, Cytoplasm, Membrane, Nucleus.
Recommended Dilutions:
  WB
  IHC-P
  FC
1:500-1:1000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: O14936 Human | O70589 Mouse | Q62915 Rat
Alternative names: CAGH39 Caki Calcium/calmodulin dependent serine protein kinase Calcium/calmodulin dependent serine protein kinase (MAGUK family) Calcium/calmodulin dependent serine protein kinase membrane associated guanylate kinase Calcium/calmodulin-dependent serine protein kinase CAMGUK CAMGUK protein CAMGUK, drosophila, homolog of casK CMG CSKP_HUMAN DXPri1 DXRib1 FGS4 FLJ22219 FLJ31914 hCASK LIN 2 Lin 2 homolog LIN2 Lin2 homolog MICPCH MRXSNA Pals3 Peripheral plasma membrane protein CASK Protein lin-2 homolog TNRC8 Trinucleotide repeat containing 8 Vertebtate LIN2 homolog
Images
EM1902-08_1.jpg Fig1: Western blot analysis of CASK on different lysates with Mouse anti-CASK antibody (EM1902-08) at 1/2,000 dilution.

Lane 1: A549-WT cell lysate
Lane 2: A549-KD CASK cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 105 kDa
Observed band size: 105 kDa

Exposure time: 3 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1902-08) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
EM1902-08_2.jpg Fig2: Western blot analysis of CASK on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-08, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Siha cell lysate
Lane 2: A549 cell lysate
EM1902-08_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-CASK antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-08, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1902-08_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-CASK antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-08, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1902-08_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CASK antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-08, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1902-08_6.jpg Fig6: Flow cytometric analysis of CASK was done on SW620 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1902-08, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa FluorTM488 Goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.