CASK Mouse Monoclonal Antibody [A1C5]
cat.: EM1902-09
| Product Type: | Mouse monoclonal IgG2a, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A1C5 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 105 kDa |
| Isotype: | IgG2a |
| Immunogen: | Recombinant protein within Human CASK aa 311-513 / 926. |
| Positive control: | SiHa cell lysate, A549 cell lysate, U-87 MG cell lysate, Neuro-2a cell lysate, human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, human prostate cancer tissue, mouse liver tissue, rat liver tissue. |
| Subcellular location: | Nucleus, Cytoplasm, Cell membrane. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:2,000 |
| Uniprot #: | SwissProt: O14936 Human | O70589 Mouse | Q62915 Rat |
| Alternative names: | CAGH39 Caki Calcium/calmodulin dependent serine protein kinase Calcium/calmodulin dependent serine protein kinase (MAGUK family) Calcium/calmodulin dependent serine protein kinase membrane associated guanylate kinase Calcium/calmodulin-dependent serine protein kinase CAMGUK CAMGUK protein CAMGUK, drosophila, homolog of casK CMG CSKP_HUMAN DXPri1 DXRib1 FGS4 FLJ22219 FLJ31914 hCASK LIN 2 Lin 2 homolog LIN2 Lin2 homolog MICPCH MRXSNA Pals3 Peripheral plasma membrane protein CASK Protein lin-2 homolog TNRC8 Trinucleotide repeat containing 8 Vertebtate LIN2 homolog |
Images
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Fig1:
Western blot analysis of CASK on different lysates with Mouse anti-CASK antibody (EM1902-09) at 1/2,000 dilution. Lane 1: SiHa cell lysate Lane 2: A549 cell lysate Lane 3: U-87 MG cell lysate Lane 4: Neuro-2a cell lysate Lane 5: Human brain tissue lysate Lane 6: Mouse brain tissue lysate Lane 7: Rat brain tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 105 kDa Observed band size: 105 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1902-09) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of CASK on different lysates with Mouse anti-CASK antibody (EM1902-09) at 1/2,000 dilution. Lane 1: A549-WT cell lysate Lane 2: A549-KD CASK cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 105 kDa Observed band size: 105 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1902-09) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Mouse anti-CASK antibody (EM1902-09) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-09) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-CASK antibody (EM1902-09) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-09) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Mouse anti-CASK antibody (EM1902-09) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-09) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.