COX2 Mouse Monoclonal Antibody [A3F8]
cat.: EM1902-11
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human, Rat
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: A3F8
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein G affinity purified.
Molecular weight: 69 kDa
Isotype: IgG1
Immunogen: Synthetic peptide within C-terminal human COX2.
Positive control: A549 cell lysates, rat spinal cord tissue lysates,rat bladder tissue, human lung carcinoma tissue, human womb tissue, A549.
Subcellular location: Microsome membrane, endoplasmic reticulum membrane.
Recommended Dilutions:
  WB
  IHC-P
  FC
1:500-1:1,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P35354 Human | P35355 Rat
Alternative names: COX 2 COX-2 COX2 Cyclooxygenase 2 Cyclooxygenase 2b Cyclooxygenase Cyclooxygenase-2 Cyclooxygenase2 EC 1.14.99.1 fj02a10 Glucocorticoid-regulated inflammatory cyclooxygenase Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase GRIPGHS hCox 2 Macrophage activation-associated marker protein P71/73 OTTHUMP00000033524 PES-2 PGG/HS PGH synthase 2 PGH2_HUMAN PGHS 2 PGHS-2 PGHS2 PHS 2 PHS II PHS2 Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) Prostaglandin endoperoxide synthase 2 Prostaglandin G/H synthase 2 Prostaglandin G/H synthase 2 precursor Prostaglandin G/H synthase and cyclooxygenase Prostaglandin G/H synthase Prostaglandin H2 synthase 2 prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) Prostaglandin-endoperoxide synthase 2 PTGS2 ptgs2a TIS10 TIS10 protein unp1239 wu:fj02a10
Images
EM1902-11_1.jpg Fig1: Western blot analysis of COX2 on A549 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-11, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
EM1902-11_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat bladder tissue using anti-COX2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-11, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1902-11_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-COX2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-11, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1902-11_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human womb tissue using anti-COX2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-11, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1902-11_5.jpg Fig5: Flow cytometric analysis of COX2 was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1902-11, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.