CD22 Mouse Monoclonal Antibody [A2G6]
cat.: EM1902-13
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | A2G6 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 95 kDa. |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human CD22 aa 20-200. |
| Positive control: | Raji cell lysate, Daudi cell lysate, Ramos cell lysate,human spleen tissue, Daudi. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:5,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P20273 Human |
| Alternative names: | B cell receptor CD22 precursor B lymphocyte cell adhesion molecule B-cell receptor CD22 B-lymphocyte cell adhesion molecule BL CAM BL-CAM BLCAM CD 22 CD22 CD22 antigen CD22 molecule CD22 protein CD22_HUMAN Lectin 2 Leu14 Lyb8 MGC130020 sialic acid binding Ig like lectin 2 Sialic acid binding immunoglobulin like lectin 2 Sialic acid-binding Ig-like lectin 2 SIGLEC 2 Siglec-2 SIGLEC2 T cell surface antigen Leu 14 T-cell surface antigen Leu-14 |
Images
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Fig1:
Western blot analysis of CD22 on different lysates with Mouse anti-CD22 antibody (EM1902-13) at 1/5,000 dilution. Lane 1: Daudi (Human Burkitt's lymphoma cell) cell lysate Lane 2: Raji (Human Burkitt's lymphoma cell) cell lysate Lane 3: Ramos (Human Burkitt's lymphoma cell) cell lysate Lane 4: Jurkat (Human T-lymphoblastic cells) cell lysate(negative) Lysates/proteins at 15 µg/Lane. Exposure time: 12 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: EM1902-13, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 95 kDa Observed band size: 140 kDa |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD22 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-13, 1/1000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Flow cytometric analysis of CD22 was done on Daudi cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1902-13, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa FluorTM488 Goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.