Anti-CD22 antibody [A2G6]
cat.: EM1902-13
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: A2G6
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/mL.
Purification: Protein G affinity purified.
Molecular weight: Predicted band size: 95 kDa.
Isotype: IgG1
Immunogen: Recombinant protein within Human CD22 aa 20-200.
Positive control: Raji cell lysate, Daudi cell lysate, human spleen tissue, Daudi.
Subcellular location: Cell membrane.
Recommended Dilutions:

Uniprot #: SwissProt: P20273 Human
Alternative names: B cell receptor CD22 precursor antibody B lymphocyte cell adhesion molecule antibody B-cell receptor CD22 antibody B-lymphocyte cell adhesion molecule antibody BL CAM antibody BL-CAM antibody BLCAM antibody CD 22 antibody CD22 antibody CD22 antigen antibody CD22 molecule antibody CD22 protein antibody CD22_HUMAN antibody Lectin 2 antibody Leu14 antibody Lyb8 antibody MGC130020 antibody sialic acid binding Ig like lectin 2 antibody Sialic acid binding immunoglobulin like lectin 2 antibody Sialic acid-binding Ig-like lectin 2 antibody SIGLEC 2 antibody Siglec-2 antibody SIGLEC2 antibody T cell surface antigen Leu 14 antibody T-cell surface antigen Leu-14 antibody
EM1902-13_1.jpg Fig1: Western blot analysis of CD22 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-13, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Raji cell lysate
Lane 2: Daudi cell lysate
EM1902-13_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD22 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-13, 1/1000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1902-13_3.jpg Fig3: Flow cytometric analysis of CD22 was done on Daudi cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1902-13, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa FluorTM488 Goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).