CD42b Mouse Monoclonal Antibody [13G2]
cat.: EM1902-15
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human
Applications: IHC-P, FC
Clonality: Monoclonal
Clone number: 13G2
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/mL
Purification: Protein A affinity purified.
Molecular weight: 72 kDa
Isotype: IgG1
Immunogen: Recombinant protein within human CD42b aa 100-350.
Positive control: human spleen tissue, Jurkat.
Subcellular location: Membrane.
Recommended Dilutions:
  IHC-P
  FC

1:100-1:500
1:50-1:100
Uniprot #: SwissProt: P07359 Human
Alternative names: Antigen CD42b alpha BDPLT1 BDPLT3 BSS CD 42b CD42b alpha CD42B CD42b antigen DBPLT3 GLYCOCALICIN Glycoprotein Ib (platelet) alpha polypeptide Glycoprotein Ibalpha GP Ib alpha GP1B GP1BA GPIb alpha GPIbA MGC34595 Platelet glycoprotein Ib alpha chain Platelet glycoprotein Ib alpha polypeptide Platelet membrane glycoprotein 1b alpha subunit VWDP
Images
EM1902-15_1.jpg Fig1: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD42b antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-15, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1902-15_2.jpg Fig2: Flow cytometric analysis of CD42b was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1902-15, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.