CD42b Mouse Monoclonal Antibody [13G2]
cat.: EM1902-15
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | 13G2 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 72 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human CD42b aa 100-350 / 652. |
| Positive control: | Human spleen tissue, human bone marrow tissue, Jurkat. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
IHC-P FC |
1:1,000 1:50-1:100 |
| Uniprot #: | SwissProt: P07359 Human |
| Alternative names: | Antigen CD42b alpha BDPLT1 BDPLT3 BSS CD 42b CD42b alpha CD42B CD42b antigen DBPLT3 GLYCOCALICIN Glycoprotein Ib (platelet) alpha polypeptide Glycoprotein Ibalpha GP Ib alpha GP1B GP1BA GPIb alpha GPIbA MGC34595 Platelet glycoprotein Ib alpha chain Platelet glycoprotein Ib alpha polypeptide Platelet membrane glycoprotein 1b alpha subunit VWDP |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-CD42b antibody (EM1902-15) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-15) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human bone marrow tissue with Mouse anti-CD42b antibody (EM1902-15) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-15) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Flow cytometric analysis of CD42b was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1902-15, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.