Anti-SARS-CoV-2 Nucleocapsid Protein antibody [A4E1]
cat.: EM1902-20
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: SARS-CoV-2
Applications: ELISA, WB
Clonality: Monoclonal
Clone number: A4E1
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4).
Concentration: 1 mg/mL.
Purification: Protein G affinity purified.
Molecular weight: 46 kDa
Isotype: IgG1
Immunogen: Recombinant SARS-CoV-2 Nucleocapsid Protein.
Positive control: ELISA: Recombinant SARS-CoV-2 Nucleocapsid Protein. WB: N-terminal His tag-tagged SARS-CoV-2 Nucleocapsid protein and inactivated native SARS-CoV-2 lysate.
Subcellular location: Virion, Host endoplasmic reticulum-Golgi intermediate compartment, Host Golgi apparatus.
Recommended Dilutions:
  ELISA
  WB

Use at an assay dependent concentration.
1:500-1:5,000
Uniprot #: SwissProt: P0DTC9
Gene ID: 1805293653
Alternative names: SARS-CoV-2 Nucleocapsid Protein SARS-CoV-2 NP nucleocapsid protein novel coronavirus N Protein novel coronavirus Nucleocapsid Protein 2019-nCoV Nucleoprotein 2019-nCoV N 2019nCoV N 2019-nCoV N Protein 2019 peak N Protein 2019-nCoV nucleocapsid protein
Images
EM1902-20_1.jpg Fig1: The binding activity of EM1902-20 with Recombinant SARS-CoV-2 Nucleocapsid protein.
Immobilized Recombinant SARS-CoV-2 nucleocapsid protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody (EM1902-20) for 1 hour at 25℃. The EC50 of EM1902-20 is 381.8 ng/ml.
EM1902-20_2.jpg Fig2: Western blot analysis of EM1902-20 with N-terminal His tag-tagged SARS-CoV-2 Nucleocapsid protein (1) and inactivated native SARS-CoV-2 lysate (2) at 20ng.
Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-20, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.